High-performance liquid chromatography of ergot alkaloids

László Szepesy, István Fehér, Gábor Szepesi, Mária Gazdag

Research output: Contribution to journalArticle

38 Citations (Scopus)


In order to develop a general method for the analysis of ergot alkaloids, the application of high-performance liquid chromatography has been investigated. The different analytical tasks involved can be solved unambiguously on two columns containing different packings (silica and a reversed-phase packing) of high efficiency. The silica packing serves for group separation and the analysis of contaminants. A rapid and simple method is presented for the screening of plant extracts. Separation of the active compounds was accomplished on reversed-phase packings and the influence of the bonded hydrocarbon groups was investigated. By using a high-efficiency microparticulate reversed-phase packing, baseline separation can be achieved under isocratic conditions for the active compounds and also the stereo-and structural isomers can be separated.

Original languageEnglish
Pages (from-to)271-280
Number of pages10
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - Feb 11 1978

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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