High-performance liquid chromatography of ergot alkaloids

L. Szepesy, István Fehér, Gábor Szepesi, Mária Gazdag

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In order to develop a general method for the analysis of ergot alkaloids, the application of high-performance liquid chromatography has been investigated. The different analytical tasks involved can be solved unambiguously on two columns containing different packings (silica and a reversed-phase packing) of high efficiency. The silica packing serves for group separation and the analysis of contaminants. A rapid and simple method is presented for the screening of plant extracts. Separation of the active compounds was accomplished on reversed-phase packings and the influence of the bonded hydrocarbon groups was investigated. By using a high-efficiency microparticulate reversed-phase packing, baseline separation can be achieved under isocratic conditions for the active compounds and also the stereo-and structural isomers can be separated.

Original languageEnglish
Pages (from-to)271-280
Number of pages10
JournalJournal of Chromatography A
Volume149
Issue numberC
DOIs
Publication statusPublished - Feb 11 1978

Fingerprint

Ergot Alkaloids
High performance liquid chromatography
Silicon Dioxide
High Pressure Liquid Chromatography
Plant Extracts
Hydrocarbons
Isomers
Screening
Impurities

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

High-performance liquid chromatography of ergot alkaloids. / Szepesy, L.; Fehér, István; Szepesi, Gábor; Gazdag, Mária.

In: Journal of Chromatography A, Vol. 149, No. C, 11.02.1978, p. 271-280.

Research output: Contribution to journalArticle

Szepesy, L. ; Fehér, István ; Szepesi, Gábor ; Gazdag, Mária. / High-performance liquid chromatography of ergot alkaloids. In: Journal of Chromatography A. 1978 ; Vol. 149, No. C. pp. 271-280.
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