High-performance liquid chromatographic-tandem mass spectrometric method for the determination of clemastine in human plasma

Viola Horváth, Antal Tolokán, Andrea Egresi, Tímea Pap, George Horvai, Katalin Balogh-Nemes, Imre Klebovich

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11 Citations (Scopus)

Abstract

A highly sensitive high-performance liquid chromatographic-tandem mass spectrometric method (HPLC-MS-MS) has been developed to quantitate clemastine in human plasma for the purpose of pharmacokinetic studies. Sample preparation was carried out by liquid-liquid extraction using deuterated clemastine as an internal standard. Chromatographic separation used a C18 reversed phase polymer column giving an extremely fast total run time of 2 min. The method was validated and used for the bioequivalence study of clemastine tablets in healthy male volunteers (n = 28). The lower limit of detection proved to be 0.01 ng/ml for clemastine.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume816
Issue number1-2
DOIs
Publication statusPublished - Feb 25 2005

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Keywords

  • Antihistamine
  • Clemastine
  • High-performance liquid chromatography-tandem mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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