High-performance liquid chromatographic study of the reduction of protected oxytocein by sodium in liquid ammonia

A. Péter, F. Lukács, K. Burger, I. Schón, M. Lów, L. Kisfaludy

Research output: Contribution to journalArticle

Abstract

A combined high-performance liquid chromatographic (HPLC)-electrochemical method was developed to investigate the mechanism of reduction of protected oxytocein by metallic sodium in liquid ammonia. The changes in the redox potential and conductivity of protected oxytosein solution provided information on the stoichiometry of the reduction. HPLC methods elaborated for the identification and selective determination of the reaction intermediates and products were used to analyse the compounds formed. This combined procedure revealed the reaction pathe of the reduction processes and permitted optimization of the experimental conditions to increase the yield of oxytocin.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalJournal of Chromatography A
Volume507
Issue numberC
DOIs
Publication statusPublished - May 16 1990

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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