High-performance liquid chromatographic separation of enantiomers of synthetic amino acids on a ristocetin A chiral stationary phase

A. Péter, Gabriella Török, Daniel W. Armstrong, Géza Tóth, Dirk Tourwé

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A macrocyclic glycopeptide, ristocetin A, was used as chiral stationary phase for the high-performance liquid chromatographic separation of enantiomers of 28 unnatural amino acids, such as analogues of phenylalanine, tyrosine and tryptophan, and analogues containing 1,2,3,4-tetrahydroisoquinoline, tetraline or 1,2,3,4-tetrahydro-2-carboline skeletons. Excellent resolutions were achieved for most of the investigated compounds by using reversed-phase or a new polar-organic mobile phase system. The conditions of separation were optimized by variation of the mobile phase composition, temperature and flow-rate. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalJournal of Chromatography A
Volume904
Issue number1
DOIs
Publication statusPublished - Dec 22 2000

Fingerprint

Carbolines
Enantiomers
Glycopeptides
Phenylalanine
Skeleton
Tryptophan
Tyrosine
Amino Acids
Temperature
Liquids
Phase composition
Flow rate
ristocetin A
1,2,3,4-tetrahydroisoquinoline

Keywords

  • Amino acids
  • Chiral stationary phases, LC
  • Enantiomer separation
  • Ristocetin A

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

High-performance liquid chromatographic separation of enantiomers of synthetic amino acids on a ristocetin A chiral stationary phase. / Péter, A.; Török, Gabriella; Armstrong, Daniel W.; Tóth, Géza; Tourwé, Dirk.

In: Journal of Chromatography A, Vol. 904, No. 1, 22.12.2000, p. 1-15.

Research output: Contribution to journalArticle

Péter, A. ; Török, Gabriella ; Armstrong, Daniel W. ; Tóth, Géza ; Tourwé, Dirk. / High-performance liquid chromatographic separation of enantiomers of synthetic amino acids on a ristocetin A chiral stationary phase. In: Journal of Chromatography A. 2000 ; Vol. 904, No. 1. pp. 1-15.
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