High-performance liquid chromatographic method for the separation of isomers of cis- and trans-2-amino-cyclopentane-1-carboxylic acid

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A method was developed for the separation of cis-(1S,2R)-, cis-(1R,2S)-, trans-(1S,2S)- and trans-(1R,2R)-2-amino-cyclopentane-1-carboxylic acids by using pre-column derivatization with the chiral derivatizing reagents 1-fluoro-2,4-dinitrophenyl-5-l-alanine amide and 2,3,4,6-tetra-O-acetyl-β-d-glucopyranosyl isothiocyanate. The method is suitable for the separaton of both diastereometric and enantiomeric pairs. The high-performance liquid chromatographic conditions (pH, eluent composition and different buffers) were varied to obtain optimal separation.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalJournal of Chromatography A
Issue number2
Publication statusPublished - Nov 3 1995


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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