β-Receptor gátlók enantiomerjeinek folyadékkromatográfiás elválasztása egy új izotiocianát típusú derivatizáló reagens segítségével

Translated title of the contribution: High-performance liquid chromatographic enantioseparation of β-blocking agents with a new isothiocyanate type chiral derivatizing agent

Á Gyéresi Á, M. Péter, F. Fülöp

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The applicability of (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate [(R,R)-DANI] as a recently developed chiral derivatizing agent for the enantioseparation of a series of β-blockers is described. The thiourea diastereomers formed were analysed by reversed-phase high-performance liquid chromatography, mixtures of water and methanol or acetonitrile being used for elution. Conditions of derivatizations (temperature, reagent excess and reaction time) were optimized. The effects of organic modifiers on the retention and separation were investigated; the diastereomers could readily be baseline-separated with methanol-containing mobile phases. The reagent allowed the detection of all four stereoisomers of labetalol, which has two chiral centres.

Original languageHungarian
Pages (from-to)138-145
Number of pages8
JournalActa Pharmaceutica Hungarica
Volume70
Issue number3-6
Publication statusPublished - 2000

Fingerprint

Methanol
Labetalol
Thiourea
Stereoisomerism
Reverse-Phase Chromatography
High Pressure Liquid Chromatography
Temperature
Water
isothiocyanic acid
acetonitrile
4-nitrophenyl

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

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title = "β-Receptor g{\'a}tl{\'o}k enantiomerjeinek folyad{\'e}kkromatogr{\'a}fi{\'a}s elv{\'a}laszt{\'a}sa egy {\'u}j izotiocian{\'a}t t{\'i}pus{\'u} derivatiz{\'a}l{\'o} reagens seg{\'i}ts{\'e}g{\'e}vel",
abstract = "The applicability of (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate [(R,R)-DANI] as a recently developed chiral derivatizing agent for the enantioseparation of a series of β-blockers is described. The thiourea diastereomers formed were analysed by reversed-phase high-performance liquid chromatography, mixtures of water and methanol or acetonitrile being used for elution. Conditions of derivatizations (temperature, reagent excess and reaction time) were optimized. The effects of organic modifiers on the retention and separation were investigated; the diastereomers could readily be baseline-separated with methanol-containing mobile phases. The reagent allowed the detection of all four stereoisomers of labetalol, which has two chiral centres.",
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year = "2000",
language = "Hungarian",
volume = "70",
pages = "138--145",
journal = "Acta Pharmaceutica Hungarica",
issn = "0001-6659",
publisher = "Magyar Gyogyszereszeti Tarsasag",
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TY - JOUR

T1 - β-Receptor gátlók enantiomerjeinek folyadékkromatográfiás elválasztása egy új izotiocianát típusú derivatizáló reagens segítségével

AU - Gyéresi Á, Á

AU - Péter, M.

AU - Fülöp, F.

PY - 2000

Y1 - 2000

N2 - The applicability of (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate [(R,R)-DANI] as a recently developed chiral derivatizing agent for the enantioseparation of a series of β-blockers is described. The thiourea diastereomers formed were analysed by reversed-phase high-performance liquid chromatography, mixtures of water and methanol or acetonitrile being used for elution. Conditions of derivatizations (temperature, reagent excess and reaction time) were optimized. The effects of organic modifiers on the retention and separation were investigated; the diastereomers could readily be baseline-separated with methanol-containing mobile phases. The reagent allowed the detection of all four stereoisomers of labetalol, which has two chiral centres.

AB - The applicability of (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate [(R,R)-DANI] as a recently developed chiral derivatizing agent for the enantioseparation of a series of β-blockers is described. The thiourea diastereomers formed were analysed by reversed-phase high-performance liquid chromatography, mixtures of water and methanol or acetonitrile being used for elution. Conditions of derivatizations (temperature, reagent excess and reaction time) were optimized. The effects of organic modifiers on the retention and separation were investigated; the diastereomers could readily be baseline-separated with methanol-containing mobile phases. The reagent allowed the detection of all four stereoisomers of labetalol, which has two chiral centres.

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