High-performance displacement chromatography of corticosteroids. Scouting for displacer and analysis of the effluent by thin-layer chromatography

Huba Kalász, Csaba Horváth

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

High-performance displacement chromatography was used for preparative scale separation of corticosterone, deoxycorticosterone and Reichstein's substance S with high-performance liquid chromatographic (HPLC) columns and instrumentation. Thin-layer chromatography (TLC) in the displacement mode was used to find an adequate carrier as well as a suitable displacer and its appropriate concentration. Separation of a mixture containing 60 mg of each component was carried out with a 500 >x 4.6 mm column packed with 5-μm silica gel. The carrier was chloroform and the displacer was 5% (v/v) diethylethanediamine in chloroform. Regeneration of the column was carried out with alcoholic acetic acid-chloroform mixtures and checked by an HPLC test. In order to evaluate the results, fractions of the column effluent were analyzed by TLC and the results were confirmed by HPLC. TLC was found to be a sufficiently accurate and highly convenient tool to determine product distribution upon separation by displacement chromatography.

Original languageEnglish
Pages (from-to)423-438
Number of pages16
JournalJournal of Chromatography A
Volume239
Issue numberC
DOIs
Publication statusPublished - Apr 30 1982

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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