Abstract
High mobility group box 1 protein (HMGB1), a nuclear protein, is a critical cytokine that mediates the response to infection, injury, and inflammation. The aim of our study was to elaborate a reliable in vitro model to investigate whether Mycobacterium bovis BCG is able to induce HMGB1 secretion from the monocytic U-937 cells. Western blot technique was applied for the detection of HMGB1 from supernatants of cells, following induction with Mycobacterium bovis BCG. Densitometric analysis revealed higher concentrations of HMGB1 in cell supernatants stimulated with BCG than in the supernatants of the control, nonstimulated cells. Further quantitation of the secreted HMGB1 was performed by ELISA. The BCG strain resulted in a higher amount of secreted HMGB1 (450 ± 44 ng/mL) than that of LPS (84 ± 12 ng/mL) or Staphylococcus aureus (150 ± 14 ng/mL). BCG and Phorbol -12-myristate -13 acetate (PMA), added together, resulted in the highest HMGB1 secretion (645 ± 125 ng/mL).The translocation of the HMGB1 towards the cytoplasm following infection of cells with BCG was demonstrated by immunofluorescence examinations. Conclusion: Our pilot experiments draw attention to the HMGB1 inducing ability of Mycobacterium bovis. Assesment of the pathophysiological role of this late cytokine in mycobacterial infections demands further in vitro and in vivo examinations.
| Original language | English |
|---|---|
| Article number | 53805 |
| Journal | Mediators of Inflammation |
| Volume | 2007 |
| DOIs | |
| Publication status | Published - 2007 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Cell Biology
Cite this
High mobility group box 1 protein induction by Mycobacterium bovis BCG. / Hofner, Péter; Seprényi, György; Miczák, A.; Buzás, Krisztina; Gyulai, Zsófia; Medzihradszky, Katalin F.; Rouhiainen, Ari; Rauvala, Heikki; Máandi, Yvette.
In: Mediators of Inflammation, Vol. 2007, 53805, 2007.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High mobility group box 1 protein induction by Mycobacterium bovis BCG
AU - Hofner, Péter
AU - Seprényi, György
AU - Miczák, A.
AU - Buzás, Krisztina
AU - Gyulai, Zsófia
AU - Medzihradszky, Katalin F.
AU - Rouhiainen, Ari
AU - Rauvala, Heikki
AU - Máandi, Yvette
PY - 2007
Y1 - 2007
N2 - High mobility group box 1 protein (HMGB1), a nuclear protein, is a critical cytokine that mediates the response to infection, injury, and inflammation. The aim of our study was to elaborate a reliable in vitro model to investigate whether Mycobacterium bovis BCG is able to induce HMGB1 secretion from the monocytic U-937 cells. Western blot technique was applied for the detection of HMGB1 from supernatants of cells, following induction with Mycobacterium bovis BCG. Densitometric analysis revealed higher concentrations of HMGB1 in cell supernatants stimulated with BCG than in the supernatants of the control, nonstimulated cells. Further quantitation of the secreted HMGB1 was performed by ELISA. The BCG strain resulted in a higher amount of secreted HMGB1 (450 ± 44 ng/mL) than that of LPS (84 ± 12 ng/mL) or Staphylococcus aureus (150 ± 14 ng/mL). BCG and Phorbol -12-myristate -13 acetate (PMA), added together, resulted in the highest HMGB1 secretion (645 ± 125 ng/mL).The translocation of the HMGB1 towards the cytoplasm following infection of cells with BCG was demonstrated by immunofluorescence examinations. Conclusion: Our pilot experiments draw attention to the HMGB1 inducing ability of Mycobacterium bovis. Assesment of the pathophysiological role of this late cytokine in mycobacterial infections demands further in vitro and in vivo examinations.
AB - High mobility group box 1 protein (HMGB1), a nuclear protein, is a critical cytokine that mediates the response to infection, injury, and inflammation. The aim of our study was to elaborate a reliable in vitro model to investigate whether Mycobacterium bovis BCG is able to induce HMGB1 secretion from the monocytic U-937 cells. Western blot technique was applied for the detection of HMGB1 from supernatants of cells, following induction with Mycobacterium bovis BCG. Densitometric analysis revealed higher concentrations of HMGB1 in cell supernatants stimulated with BCG than in the supernatants of the control, nonstimulated cells. Further quantitation of the secreted HMGB1 was performed by ELISA. The BCG strain resulted in a higher amount of secreted HMGB1 (450 ± 44 ng/mL) than that of LPS (84 ± 12 ng/mL) or Staphylococcus aureus (150 ± 14 ng/mL). BCG and Phorbol -12-myristate -13 acetate (PMA), added together, resulted in the highest HMGB1 secretion (645 ± 125 ng/mL).The translocation of the HMGB1 towards the cytoplasm following infection of cells with BCG was demonstrated by immunofluorescence examinations. Conclusion: Our pilot experiments draw attention to the HMGB1 inducing ability of Mycobacterium bovis. Assesment of the pathophysiological role of this late cytokine in mycobacterial infections demands further in vitro and in vivo examinations.
UR - http://www.scopus.com/inward/record.url?scp=36949021094&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36949021094&partnerID=8YFLogxK
U2 - 10.1155/2007/53805
DO - 10.1155/2007/53805
M3 - Article
VL - 2007
JO - Mediators of Inflammation
JF - Mediators of Inflammation
SN - 0962-9351
M1 - 53805
ER -