High mobility group box 1 protein induction by Mycobacterium bovis BCG

Péter Hofner, György Seprényi, A. Miczák, Krisztina Buzás, Zsófia Gyulai, Katalin F. Medzihradszky, Ari Rouhiainen, Heikki Rauvala, Yvette Máandi

Research output: Contribution to journalArticle

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Abstract

High mobility group box 1 protein (HMGB1), a nuclear protein, is a critical cytokine that mediates the response to infection, injury, and inflammation. The aim of our study was to elaborate a reliable in vitro model to investigate whether Mycobacterium bovis BCG is able to induce HMGB1 secretion from the monocytic U-937 cells. Western blot technique was applied for the detection of HMGB1 from supernatants of cells, following induction with Mycobacterium bovis BCG. Densitometric analysis revealed higher concentrations of HMGB1 in cell supernatants stimulated with BCG than in the supernatants of the control, nonstimulated cells. Further quantitation of the secreted HMGB1 was performed by ELISA. The BCG strain resulted in a higher amount of secreted HMGB1 (450 ± 44 ng/mL) than that of LPS (84 ± 12 ng/mL) or Staphylococcus aureus (150 ± 14 ng/mL). BCG and Phorbol -12-myristate -13 acetate (PMA), added together, resulted in the highest HMGB1 secretion (645 ± 125 ng/mL).The translocation of the HMGB1 towards the cytoplasm following infection of cells with BCG was demonstrated by immunofluorescence examinations. Conclusion: Our pilot experiments draw attention to the HMGB1 inducing ability of Mycobacterium bovis. Assesment of the pathophysiological role of this late cytokine in mycobacterial infections demands further in vitro and in vivo examinations.

Original languageEnglish
Article number53805
JournalMediators of Inflammation
Volume2007
DOIs
Publication statusPublished - 2007

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HMGB1 Protein
Mycobacterium bovis
Infection
Cytokines
Nuclear Proteins
Fluorescent Antibody Technique
Staphylococcus aureus
Cytoplasm
Acetates
Western Blotting
Enzyme-Linked Immunosorbent Assay
Inflammation

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Hofner, P., Seprényi, G., Miczák, A., Buzás, K., Gyulai, Z., Medzihradszky, K. F., ... Máandi, Y. (2007). High mobility group box 1 protein induction by Mycobacterium bovis BCG. Mediators of Inflammation, 2007, [53805]. https://doi.org/10.1155/2007/53805

High mobility group box 1 protein induction by Mycobacterium bovis BCG. / Hofner, Péter; Seprényi, György; Miczák, A.; Buzás, Krisztina; Gyulai, Zsófia; Medzihradszky, Katalin F.; Rouhiainen, Ari; Rauvala, Heikki; Máandi, Yvette.

In: Mediators of Inflammation, Vol. 2007, 53805, 2007.

Research output: Contribution to journalArticle

Hofner, P, Seprényi, G, Miczák, A, Buzás, K, Gyulai, Z, Medzihradszky, KF, Rouhiainen, A, Rauvala, H & Máandi, Y 2007, 'High mobility group box 1 protein induction by Mycobacterium bovis BCG', Mediators of Inflammation, vol. 2007, 53805. https://doi.org/10.1155/2007/53805
Hofner, Péter ; Seprényi, György ; Miczák, A. ; Buzás, Krisztina ; Gyulai, Zsófia ; Medzihradszky, Katalin F. ; Rouhiainen, Ari ; Rauvala, Heikki ; Máandi, Yvette. / High mobility group box 1 protein induction by Mycobacterium bovis BCG. In: Mediators of Inflammation. 2007 ; Vol. 2007.
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