High level of field resistance of transgenic tobaccos induced by integrated potato virus Y coat protein gene

R. Józsa, Z. Stasevski, E. Balázs

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The coat protein gene (CP) from a highly virulent, necrotic and dominant strain of potato virus Y (PVY) originated from the Hungarian flora has been engineered via Agrobacterium infection into different Hungarian tobacco breeding lines and cultivars. The integration of the CP was confirmed by polymerase chain reaction (PCR) using genomic preparations. The transcription and the expression of the integrated CP gene were detected by Northern and Western analysis. The pathogen-derived resistance was demonstrated by inoculation of the RI progeny of transformation plants with purified PVY. The efficiency of the protection varied between different transgenic tobaccos ranging from complete to no protection. The challenge infection of the plants was monitored by dot blot hybridisation at different intervals after mechanical inoculation. Western blot analysis showed that there is no correlation between the level of expressed CP and the extent of resistance. From tobacco cultivars namely Virgin D, Stamm C2 and Hevesi 11, 38, 55 and 23 transformants were obtained, respectively. After several years of greenhouse experiments, only the extreme resistant tobaccos were planted field under the special licence, given by the competent authority. In field conditions, transgenic tobacco varieties showed extreme resistance against natural infection of PVY.

Original languageEnglish
Pages (from-to)311-316
Number of pages6
JournalActa Phytopathologica et Entomologica Hungarica
Volume37
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Potato virus Y
coat proteins
tobacco
genetically modified organisms
genes
infection
cultivars
breeding lines
nucleic acid hybridization
Agrobacterium
greenhouse experimentation
Western blotting
transcription (genetics)
flora
polymerase chain reaction
genomics
pathogens

Keywords

  • Field resistance
  • Potato virus Y
  • Transgenic tobacco

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

High level of field resistance of transgenic tobaccos induced by integrated potato virus Y coat protein gene. / Józsa, R.; Stasevski, Z.; Balázs, E.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 37, No. 4, 2002, p. 311-316.

Research output: Contribution to journalArticle

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