High level functional expression of the ABCG2 multidrug transporter in undifferentiated human embryonic stem cells

Ágota Apáti, Tamás I. Orbán, Nóra Varga, Andrea Németh, Anita Schamberger, Virág Krizsik, Boglárka Erdélyi-Belle, László Homolya, György Várady, Rita Padányi, Éva Karászi, Evelien W.M. Kemna, Katalin Német, Balázs Sarkadi

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64 Citations (Scopus)

Abstract

Expression of multidrug resistance ABC transporters has been suggested as a functional marker and chemoprotective element in early human progenitor cell types. In this study we examined the expression and function of the key multidrug-ABC transporters, ABCB1, ABCC1 and ABCG2 in two human embryonic stem (HuES) cell lines. We detected a high level ABCG2 expression in the undifferentiated HuES cells, while the expression of this protein significantly decreased during early cell differentiation. ABCG2 in HuES cells provided protection against mitoxantrone toxicity, with a drug-stimulated overexpression of the transporter. No significant expression of ABCB1/ABCC1 was found either in the undifferentiated or partially differentiated HuES cells. Examination of the ABCG2 mRNA in HuES cells indicated the use of selected promoter sites and a truncated 3′ untranslated region, suggesting a functionally distinct regulation of this transporter in undifferentiated stem cells. The selective expression of the ABCG2 multidrug transporter indicates that ABCG2 can be applied as a marker for undifferentiated HuES cells. Moreover, protection of embryonic stem cells against xenobiotics and endobiotics may depend on ABCG2 expression and regulation.

Original languageEnglish
Pages (from-to)2700-2709
Number of pages10
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1778
Issue number12
DOIs
Publication statusPublished - Dec 1 2008

Keywords

  • ABCB1
  • ABCC1
  • ABCG2
  • Human embryonic stem cells
  • Multidrug resistance
  • Stem cell differentiation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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  • Cite this

    Apáti, Á., Orbán, T. I., Varga, N., Németh, A., Schamberger, A., Krizsik, V., Erdélyi-Belle, B., Homolya, L., Várady, G., Padányi, R., Karászi, É., Kemna, E. W. M., Német, K., & Sarkadi, B. (2008). High level functional expression of the ABCG2 multidrug transporter in undifferentiated human embryonic stem cells. Biochimica et Biophysica Acta - Biomembranes, 1778(12), 2700-2709. https://doi.org/10.1016/j.bbamem.2008.08.010