High-frequency generation and characterization of intergeneric hybrids and haploids from new wheat-barley crosses

Dávid Polgári, András Cseh, Éva Szakács, Katalin Jäger, Márta Molnár-Láng, László Sági

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4 Citations (Scopus)

Abstract

Key message: Hybrid plants and a high frequency of maternal haploids were obtained using an efficient wheat-barley hybridization system (with new genotype combinations) and confirmed by several cytological and molecular tools. An efficient hybridization system between wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) is presented on the basis of three new genotype combinations. A particularly high, 14 % frequency of plant regeneration per florets was achieved in the wheat-barley genotype combination of 'Sichuan' × 'Morex'. The genome composition in 42 of the 95 plants regenerated by embryo rescue was determined using ploidy analysis, genomic in situ hybridization and the application of chromosome arm-specific molecular markers (SSR and STS). A high overall frequency (76 %) of maternal (wheat) haploids was observed in all the tests for all three cross combinations. A major implication of this observation is that this new hybridization system represents a useful tool to study the mechanism of uniparental chromosome elimination in cereals.

Original languageEnglish
Pages (from-to)1323-1331
Number of pages9
JournalPlant Cell Reports
Volume33
Issue number8
DOIs
Publication statusPublished - Aug 2014

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Keywords

  • Cereals
  • DNA marker
  • GISH
  • Ultrawide cross
  • Uniparental chromosome elimination

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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