High frequency callus formation and regeneration of fertile plants from haploid cell suspensions derived from anther culture in wheat (Triticum aestivum L.)

Guang Yuan He, Emese Korbuly, B. Barnabás

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Regenerable, embryogenic haploid cell suspensions were initiated and established from pollen calli of two cultivated wheat genotypes, Xu Zhou 211 and Mv 16. Repeated callus and cell selection during the culture procedure led to stable haploid suspensions consisting of fine clusters each containing 20-50 cells. These suspensions were able to maintain their morphogenic ability during 8 months of subculture. Two fertile, seed-producing plants were successfully grown from an 8-month-old suspension of Xu Zhou 211. The experimental system holds promise for use in haploid protoplast isolation and genetic manipulation.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalPlant Science
Volume90
Issue number1
DOIs
Publication statusPublished - 1993

Fingerprint

anther culture
Haploidy
callus formation
Bony Callus
Plant Cells
haploidy
cell suspension culture
Triticum
Regeneration
Suspensions
Triticum aestivum
wheat
callus
genetic engineering
protoplasts
Protoplasts
Pollen
pollen
cells
Seed

Keywords

  • anther culture
  • fertile dihaploid plant
  • haploid suspension
  • Triticum aestivum L.

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

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abstract = "Regenerable, embryogenic haploid cell suspensions were initiated and established from pollen calli of two cultivated wheat genotypes, Xu Zhou 211 and Mv 16. Repeated callus and cell selection during the culture procedure led to stable haploid suspensions consisting of fine clusters each containing 20-50 cells. These suspensions were able to maintain their morphogenic ability during 8 months of subculture. Two fertile, seed-producing plants were successfully grown from an 8-month-old suspension of Xu Zhou 211. The experimental system holds promise for use in haploid protoplast isolation and genetic manipulation.",
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T1 - High frequency callus formation and regeneration of fertile plants from haploid cell suspensions derived from anther culture in wheat (Triticum aestivum L.)

AU - He, Guang Yuan

AU - Korbuly, Emese

AU - Barnabás, B.

PY - 1993

Y1 - 1993

N2 - Regenerable, embryogenic haploid cell suspensions were initiated and established from pollen calli of two cultivated wheat genotypes, Xu Zhou 211 and Mv 16. Repeated callus and cell selection during the culture procedure led to stable haploid suspensions consisting of fine clusters each containing 20-50 cells. These suspensions were able to maintain their morphogenic ability during 8 months of subculture. Two fertile, seed-producing plants were successfully grown from an 8-month-old suspension of Xu Zhou 211. The experimental system holds promise for use in haploid protoplast isolation and genetic manipulation.

AB - Regenerable, embryogenic haploid cell suspensions were initiated and established from pollen calli of two cultivated wheat genotypes, Xu Zhou 211 and Mv 16. Repeated callus and cell selection during the culture procedure led to stable haploid suspensions consisting of fine clusters each containing 20-50 cells. These suspensions were able to maintain their morphogenic ability during 8 months of subculture. Two fertile, seed-producing plants were successfully grown from an 8-month-old suspension of Xu Zhou 211. The experimental system holds promise for use in haploid protoplast isolation and genetic manipulation.

KW - anther culture

KW - fertile dihaploid plant

KW - haploid suspension

KW - Triticum aestivum L.

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