High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes.

M. A. Jafari, J. Kiss, J. Gergácz, L. Heszky

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to develop a simple and feasible approach to achieve high frequency plant regeneration for protoplast isolation and transformation experiments, a method was elaborated by using a new type of explant (petiole segments) for the four Populus nigra genotypes. Callus initiation from the petioles took place on N6 medium containing 2,4-D (0.1-1 mg/l). The highest rate of callus initiation (100%) was achieved when the basic medium was supplemented with 0.5 mg/l of 2,4-D, in all tested genotypes. For shoot regeneration, calli were transferred to MS and WPM medium supplemented with BA (1.0-2.5 mg/l) and NAA (0.2 mg/l). Multiple shoot regeneration was observed in each shoot induction medium. The highest rate of shoot regeneration (6.83 shoot/callus) was observed on MS medium containing 2.5 mg/l BA and 0.2 mg/l NAA. The results showed highly significant differences between the media. There was no significant difference between the genotypes and genotype x medium interaction.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalActa Biologica Hungarica
Volume46
Issue number1
Publication statusPublished - 1995

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2,4-Dichlorophenoxyacetic Acid
Bony Callus
Regeneration
callus
genotype
shoot
regeneration
Genotype
shoots
2,4 dichlorophenoxyacetic acid
neutron activation analysis
2,4-D
Populus
Protoplasts
Populus nigra
petioles
Experiments
protoplasts
explants
experiment

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes. / Jafari, M. A.; Kiss, J.; Gergácz, J.; Heszky, L.

In: Acta Biologica Hungarica, Vol. 46, No. 1, 1995, p. 51-59.

Research output: Contribution to journalArticle

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