High efficiency adventive embryogenesis on somatic embryos of anther, filament and immature proembryo origin in horse-chestnut (Aesculus hippocastanum L.) tissue culture

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Adventive embryogenesis was successfully induced in cultures of zygotic and somatic embryos on MS medium supplemented with BA and NAA. A procedure has been proved successful for the in vitro multiplication of somatic embryos regenerated at low frequencies from filament and callus cultures. The occurrence and rate of adventive embryogenesis did not depend on the origin of the primary embryos (zygotic and somatic), but did depend on the developmental stage. Primary embryos are capable of embryogenesis in each of the different phases of embryogenesis, though the rate is different. BA concentrations of 22-44 μM increased the rate of adventive embryogenesis and accelerated the development of embryos. The highest proliferation rate (22-25x/5 weeks) was achieved at hormone concentrations of 44 μM BA and 5.4 μM NAA.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Volume30
Issue number1
DOIs
Publication statusPublished - Jul 1992

Fingerprint

Aesculus
Aesculus hippocastanum
somatic embryos
anthers
Embryonic Development
tissue culture
embryogenesis
Embryonic Structures
immatures
embryo (plant)
Bony Callus
callus culture
hormones
developmental stages
Hormones

Keywords

  • adventive embryogenesis
  • anther culture
  • filament culture
  • horse-chestnut
  • somatic embryogenesis

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

Cite this

@article{8ef09476d07d410db0c4c02e953fbac0,
title = "High efficiency adventive embryogenesis on somatic embryos of anther, filament and immature proembryo origin in horse-chestnut (Aesculus hippocastanum L.) tissue culture",
abstract = "Adventive embryogenesis was successfully induced in cultures of zygotic and somatic embryos on MS medium supplemented with BA and NAA. A procedure has been proved successful for the in vitro multiplication of somatic embryos regenerated at low frequencies from filament and callus cultures. The occurrence and rate of adventive embryogenesis did not depend on the origin of the primary embryos (zygotic and somatic), but did depend on the developmental stage. Primary embryos are capable of embryogenesis in each of the different phases of embryogenesis, though the rate is different. BA concentrations of 22-44 μM increased the rate of adventive embryogenesis and accelerated the development of embryos. The highest proliferation rate (22-25x/5 weeks) was achieved at hormone concentrations of 44 μM BA and 5.4 μM NAA.",
keywords = "adventive embryogenesis, anther culture, filament culture, horse-chestnut, somatic embryogenesis",
author = "J. Kiss and L. Heszky and E. Kiss and G. Gyulai",
year = "1992",
month = "7",
doi = "10.1007/BF00040001",
language = "English",
volume = "30",
pages = "59--64",
journal = "Plant Cell, Tissue and Organ Culture",
issn = "0167-6857",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - High efficiency adventive embryogenesis on somatic embryos of anther, filament and immature proembryo origin in horse-chestnut (Aesculus hippocastanum L.) tissue culture

AU - Kiss, J.

AU - Heszky, L.

AU - Kiss, E.

AU - Gyulai, G.

PY - 1992/7

Y1 - 1992/7

N2 - Adventive embryogenesis was successfully induced in cultures of zygotic and somatic embryos on MS medium supplemented with BA and NAA. A procedure has been proved successful for the in vitro multiplication of somatic embryos regenerated at low frequencies from filament and callus cultures. The occurrence and rate of adventive embryogenesis did not depend on the origin of the primary embryos (zygotic and somatic), but did depend on the developmental stage. Primary embryos are capable of embryogenesis in each of the different phases of embryogenesis, though the rate is different. BA concentrations of 22-44 μM increased the rate of adventive embryogenesis and accelerated the development of embryos. The highest proliferation rate (22-25x/5 weeks) was achieved at hormone concentrations of 44 μM BA and 5.4 μM NAA.

AB - Adventive embryogenesis was successfully induced in cultures of zygotic and somatic embryos on MS medium supplemented with BA and NAA. A procedure has been proved successful for the in vitro multiplication of somatic embryos regenerated at low frequencies from filament and callus cultures. The occurrence and rate of adventive embryogenesis did not depend on the origin of the primary embryos (zygotic and somatic), but did depend on the developmental stage. Primary embryos are capable of embryogenesis in each of the different phases of embryogenesis, though the rate is different. BA concentrations of 22-44 μM increased the rate of adventive embryogenesis and accelerated the development of embryos. The highest proliferation rate (22-25x/5 weeks) was achieved at hormone concentrations of 44 μM BA and 5.4 μM NAA.

KW - adventive embryogenesis

KW - anther culture

KW - filament culture

KW - horse-chestnut

KW - somatic embryogenesis

UR - http://www.scopus.com/inward/record.url?scp=0001912959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001912959&partnerID=8YFLogxK

U2 - 10.1007/BF00040001

DO - 10.1007/BF00040001

M3 - Article

VL - 30

SP - 59

EP - 64

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -