High-dose methylprednisolone influences the physiology and virulence of Candida albicans ambiguously and enhances the candidacidal activity of the polyene antibiotic amphotericin B and the superoxide-generating agent menadione

Ágnes Gyetvai, T. Emri, Andrea Fekete, Z. Varga, Zoltán Gazdag, M. Pesti, J. Belágyi, L. Emődy, I. Pócsi, Béla Lenkey

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although exposure of Candida albicans cells to high-dose (4 mM) methylprednisolone stimulated microbial growth, germination rate in serum and phospholipase release, it also promoted the recognition of C. albicans cells by polymorphonuclear leukocytes. Pretreatment of C. albicans cells with methylprednisolone did not result in any increase in the pathogenicity of the fungus in intraperitoneal and intravenous mouse assays. Therefore, the virulence of C. albicans is unlikely to increase in patients treated with comparably high-dose methylprednisolone on skin and mucosal membranes. Methylprednisolone treatments also increased the production of conjugated dienes and thiobarbituric acid-reactive substances, and the menadione sensitivity of C. albicans cells, which can be explained by a significant decrease in the specific activities of several antioxidant enzymes. The combination of methylprednisolone with oxidants, e.g. in topical applications, may be of clinical importance when the predisposition to candidiasis is high. Methylprednisolone treatments negatively affected membrane fluidity and decreased the antifungal effects of both the polyene antibiotic nystatin and the ergosterol biosynthesis inhibitor lovastatin, and also enhanced the deleterious effects of the polyene antimycotic amphotericin B on C. albicans cells. These corticosteroid-polyene drug interactions should be considered in the treatment of C. albicans infections in patients with prolonged topical application of corticosteroids.

Original languageEnglish
Pages (from-to)265-275
Number of pages11
JournalFEMS Yeast Research
Volume7
Issue number2
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Polyenes
Vitamin K 3
Methylprednisolone
Amphotericin B
Candida albicans
Superoxides
Virulence
Anti-Bacterial Agents
Adrenal Cortex Hormones
Ergosterol
Nystatin
Lovastatin
Membrane Fluidity
Thiobarbituric Acid Reactive Substances
Phospholipases
Candidiasis
Germination
Drug Interactions
Oxidants
Neutrophils

Keywords

  • Amphotericin B
  • Candida albicans
  • Menadione
  • Methylprednisolone
  • Nystatin
  • Virulence factors

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

@article{4fb993f7be3944cc8fb868a8dd3aa201,
title = "High-dose methylprednisolone influences the physiology and virulence of Candida albicans ambiguously and enhances the candidacidal activity of the polyene antibiotic amphotericin B and the superoxide-generating agent menadione",
abstract = "Although exposure of Candida albicans cells to high-dose (4 mM) methylprednisolone stimulated microbial growth, germination rate in serum and phospholipase release, it also promoted the recognition of C. albicans cells by polymorphonuclear leukocytes. Pretreatment of C. albicans cells with methylprednisolone did not result in any increase in the pathogenicity of the fungus in intraperitoneal and intravenous mouse assays. Therefore, the virulence of C. albicans is unlikely to increase in patients treated with comparably high-dose methylprednisolone on skin and mucosal membranes. Methylprednisolone treatments also increased the production of conjugated dienes and thiobarbituric acid-reactive substances, and the menadione sensitivity of C. albicans cells, which can be explained by a significant decrease in the specific activities of several antioxidant enzymes. The combination of methylprednisolone with oxidants, e.g. in topical applications, may be of clinical importance when the predisposition to candidiasis is high. Methylprednisolone treatments negatively affected membrane fluidity and decreased the antifungal effects of both the polyene antibiotic nystatin and the ergosterol biosynthesis inhibitor lovastatin, and also enhanced the deleterious effects of the polyene antimycotic amphotericin B on C. albicans cells. These corticosteroid-polyene drug interactions should be considered in the treatment of C. albicans infections in patients with prolonged topical application of corticosteroids.",
keywords = "Amphotericin B, Candida albicans, Menadione, Methylprednisolone, Nystatin, Virulence factors",
author = "{\'A}gnes Gyetvai and T. Emri and Andrea Fekete and Z. Varga and Zolt{\'a}n Gazdag and M. Pesti and J. Bel{\'a}gyi and L. Emődy and I. P{\'o}csi and B{\'e}la Lenkey",
year = "2007",
month = "3",
doi = "10.1111/j.1567-1364.2006.00179.x",
language = "English",
volume = "7",
pages = "265--275",
journal = "FEMS Yeast Research",
issn = "1567-1356",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - High-dose methylprednisolone influences the physiology and virulence of Candida albicans ambiguously and enhances the candidacidal activity of the polyene antibiotic amphotericin B and the superoxide-generating agent menadione

AU - Gyetvai, Ágnes

AU - Emri, T.

AU - Fekete, Andrea

AU - Varga, Z.

AU - Gazdag, Zoltán

AU - Pesti, M.

AU - Belágyi, J.

AU - Emődy, L.

AU - Pócsi, I.

AU - Lenkey, Béla

PY - 2007/3

Y1 - 2007/3

N2 - Although exposure of Candida albicans cells to high-dose (4 mM) methylprednisolone stimulated microbial growth, germination rate in serum and phospholipase release, it also promoted the recognition of C. albicans cells by polymorphonuclear leukocytes. Pretreatment of C. albicans cells with methylprednisolone did not result in any increase in the pathogenicity of the fungus in intraperitoneal and intravenous mouse assays. Therefore, the virulence of C. albicans is unlikely to increase in patients treated with comparably high-dose methylprednisolone on skin and mucosal membranes. Methylprednisolone treatments also increased the production of conjugated dienes and thiobarbituric acid-reactive substances, and the menadione sensitivity of C. albicans cells, which can be explained by a significant decrease in the specific activities of several antioxidant enzymes. The combination of methylprednisolone with oxidants, e.g. in topical applications, may be of clinical importance when the predisposition to candidiasis is high. Methylprednisolone treatments negatively affected membrane fluidity and decreased the antifungal effects of both the polyene antibiotic nystatin and the ergosterol biosynthesis inhibitor lovastatin, and also enhanced the deleterious effects of the polyene antimycotic amphotericin B on C. albicans cells. These corticosteroid-polyene drug interactions should be considered in the treatment of C. albicans infections in patients with prolonged topical application of corticosteroids.

AB - Although exposure of Candida albicans cells to high-dose (4 mM) methylprednisolone stimulated microbial growth, germination rate in serum and phospholipase release, it also promoted the recognition of C. albicans cells by polymorphonuclear leukocytes. Pretreatment of C. albicans cells with methylprednisolone did not result in any increase in the pathogenicity of the fungus in intraperitoneal and intravenous mouse assays. Therefore, the virulence of C. albicans is unlikely to increase in patients treated with comparably high-dose methylprednisolone on skin and mucosal membranes. Methylprednisolone treatments also increased the production of conjugated dienes and thiobarbituric acid-reactive substances, and the menadione sensitivity of C. albicans cells, which can be explained by a significant decrease in the specific activities of several antioxidant enzymes. The combination of methylprednisolone with oxidants, e.g. in topical applications, may be of clinical importance when the predisposition to candidiasis is high. Methylprednisolone treatments negatively affected membrane fluidity and decreased the antifungal effects of both the polyene antibiotic nystatin and the ergosterol biosynthesis inhibitor lovastatin, and also enhanced the deleterious effects of the polyene antimycotic amphotericin B on C. albicans cells. These corticosteroid-polyene drug interactions should be considered in the treatment of C. albicans infections in patients with prolonged topical application of corticosteroids.

KW - Amphotericin B

KW - Candida albicans

KW - Menadione

KW - Methylprednisolone

KW - Nystatin

KW - Virulence factors

UR - http://www.scopus.com/inward/record.url?scp=33846954382&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846954382&partnerID=8YFLogxK

U2 - 10.1111/j.1567-1364.2006.00179.x

DO - 10.1111/j.1567-1364.2006.00179.x

M3 - Article

VL - 7

SP - 265

EP - 275

JO - FEMS Yeast Research

JF - FEMS Yeast Research

SN - 1567-1356

IS - 2

ER -