High-copy-number derivatives of the plasmid cloning vector pBR322

I. Boros, G. Pósfai, Pál Venetianer

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

A stable copy-number mutant of a pBR322-derived plasmid was isolated. The mutation was found to be a single G → T transversion located near the 3' end of a DNA segment coding for the regulatory RNA I. The resulting copy number for this plasmid is approx. 1000 per cell or 65 % of total cellular DNA. Several cloning vectors have been constructed from this copy-number mutant and their practical application is discussed.

Original languageEnglish
Pages (from-to)257-260
Number of pages4
JournalGene
Volume30
Issue number1-3
DOIs
Publication statusPublished - 1984

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Genetic Vectors
Plasmids
DNA
Mutation
RNA I

Keywords

  • Polylinker
  • Recombinant DNA
  • regulatory RNA I
  • transcription termination
  • transversion mutation

ASJC Scopus subject areas

  • Genetics

Cite this

High-copy-number derivatives of the plasmid cloning vector pBR322. / Boros, I.; Pósfai, G.; Venetianer, Pál.

In: Gene, Vol. 30, No. 1-3, 1984, p. 257-260.

Research output: Contribution to journalArticle

Boros, I. ; Pósfai, G. ; Venetianer, Pál. / High-copy-number derivatives of the plasmid cloning vector pBR322. In: Gene. 1984 ; Vol. 30, No. 1-3. pp. 257-260.
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