High-affinity iron permease (FTR1) gene sequence-based molecular identification of clinically important Zygomycetes

I. Nyilasi, T. Papp, Á Csernetics, K. Krizsán, E. Nagy, C. Vágvölgyi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The clinical importance of zygomycosis, an emerging and frequently fatal mycotic disease, has increased during recent years. This report describes an identification method based on PCR amplification and sequencing of the high-affinity iron permease. 1 gene (FTR1). Primers and amplification protocols were established and tested for the identification of Rhizopus oryzae, Rhizopus microsporus var. rhizopodiformis, R. microsporus var. oligosporus, Rhizopus schipperae, Rhizopus niveus and Rhizopus stolonifer. Rhizomucor and Syncephalastrum could be identified at the genus level. PCR-restriction fragment length polymorphism analysis of the amplified gene fragment using Alu I digestion distinguished three subgroups among the R. oryzae isolates.

Original languageEnglish
Pages (from-to)393-397
Number of pages5
JournalClinical Microbiology and Infection
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Rhizopus
Membrane Transport Proteins
Iron
Genes
Rhizomucor
Zygomycosis
Polymerase Chain Reaction
Restriction Fragment Length Polymorphisms
Digestion

Keywords

  • Identification, iron permease 1 gene
  • Mucorales
  • PCR-restriction fragment length polymorphism analysis
  • Rhizopus
  • Zygomycosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

High-affinity iron permease (FTR1) gene sequence-based molecular identification of clinically important Zygomycetes. / Nyilasi, I.; Papp, T.; Csernetics, Á; Krizsán, K.; Nagy, E.; Vágvölgyi, C.

In: Clinical Microbiology and Infection, Vol. 14, No. 4, 04.2008, p. 393-397.

Research output: Contribution to journalArticle

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