Hid can induce, but is not required for autophagy in polyploid larval Drosophila tissues

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The major cell death pathways are apoptosis and autophagy-type cell death in Drosophila. Overexpression of proapoptotic genes in developing imaginal tissues leads to the activation of caspases and apoptosis, but most of them show no effect on the polytenic cells of the fat body during the last larval stage. Surprisingly, overexpression of Hid induces caspase-independent autophagy in the fat body, as well as in most other larval tissues tested. Hid mutation results in inhibition of salivary gland cell death, but the disintegration of the larval midgut is not affected. Electron microscopy shows that autophagy is normally induced in fat body, midgut and salivary gland cells of homozygous mutant larvae, suggesting that Hid is not required for autophagy itself. Constitutive expression of the caspase inhibitor p35 produces identical phenotypes. Our results show that the large, post-mitotic larval cells do not react or activate autophagy in response to the same strong apoptotic stimuli that trigger apoptosis in small, mitotically active imaginal disc cells.

Original languageEnglish
Pages (from-to)491-502
Number of pages12
JournalEuropean Journal of Cell Biology
Volume84
Issue number4
DOIs
Publication statusPublished - Apr 22 2005

Fingerprint

Polyploidy
Autophagy
Drosophila
Fat Body
Cell Death
Apoptosis
Caspases
Salivary Glands
Imaginal Discs
Caspase Inhibitors
Larva
Electron Microscopy
Phenotype
Mutation
Genes

Keywords

  • Apoptosis
  • Autophagy
  • Cell death
  • Drosophila
  • Fat body
  • Hid

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Hid can induce, but is not required for autophagy in polyploid larval Drosophila tissues. / Juhász, G.; Sass, M.

In: European Journal of Cell Biology, Vol. 84, No. 4, 22.04.2005, p. 491-502.

Research output: Contribution to journalArticle

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