Hg2+ reacts with different components of the NADPH:protochlorophyllide oxidoreductase macrodomains

K. Solymosi, K. Lenti, B. Myśliwa-Kurdziel, J. Fidy, K. Strzałka, B. Böddi

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25 Citations (Scopus)

Abstract

The molecular background of Hg2+-induced inhibition of protochlorophyllide (Pchlide) photoreduction was investigated in homogenates of dark-grown wheat leaves. Our earlier work showed that 15 min incubation with 10-2 M Hg2+ completely inhibits the activity of NADPH: Pchlide oxidoreductase (Lenti et al., 2002). Detailed analysis of spectra recorded at 10 K indicated the appearance of emission bands at 638 and 650 nm, which are characteristic for NADP+-Pchlide complexes. Fluorescence emission spectra recorded with different excitation wavelengths, fluorescence lifetime measurements and the analysis of acetone extractions revealed that Hg2+ can also react directly with Pchlide, resulting in protopheophorbide formation. At 10-3 M Hg2+, the phototransformation was complete but the blue shift of the chlorophyllide emission band speeded up remarkably. This indicates oxidation of the NADPH molecules that have a structural role in keeping together the etioplast inner membrane components. We suggest a complex model for the Hg2+ effect: depending on concentration it can react with any components of the NADPH : Pchlide oxidoreductase macrodomains.

Original languageEnglish
Pages (from-to)358-368
Number of pages11
JournalPlant Biology
Volume6
Issue number3
DOIs
Publication statusPublished - May 2004

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Keywords

  • Activity loss
  • Hg
  • Mercury
  • NADPH
  • Protochlorophyllide
  • Protopheophorbide
  • Shibata shift

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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