Hexose phosphorylation and the putative calcium channel component Mid1p are required for the hexose-induced transient elevation of cytosolic calcium response in Saccharomyces cerevisiae

Margit Tökés-Füzesi, David M. Bedwell, I. Repa, Katalin Sipos, B. Sümegi, András Rab, A. Miseta

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Saccharomyces cerevisiae responds to environmental stimuli such as an exposure to pheromone or to hexoses after carbon source limitation with a transient elevation of cytosolic calcium (TECC) response. In this study, we examined whether hexose transport and phosphorylation are necessary for the TECC response. We found that a mutant strain lacking most of the known hexose transporters was unable to carry out the TECC response when exposed to glucose. A mutant strain that lacked the ability to phosphorylate glucose was unable to respond to glucose addition, but displayed a normal TECC response after the addition of galactose. These results indicate that hexose uptake and phosphorylation are required to trigger the hexose-induced TECC response. We also found that the TECC response was significantly smaller than normal when the level of environmental calcium was reduced, and was abolished in a mid1 mutant that lacked a subunit of the high-affinity calcium channel of the yeast plasma membrane. These results indicate that most or all of the TECC response is mediated by an influx of calcium from the extracellular space. Our results indicate that this transient increase in plasma membrane calcium permeability may be linked to the accumulation of Glc-1-P (or a related glucose metabolite) in yeast.

Original languageEnglish
Pages (from-to)1299-1308
Number of pages10
JournalMolecular Microbiology
Volume44
Issue number5
DOIs
Publication statusPublished - 2002

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Hexoses
Calcium Channels
Saccharomyces cerevisiae
Phosphorylation
Calcium
Glucose
Monosaccharide Transport Proteins
Yeasts
Cell Membrane
Pheromones
Extracellular Space
Galactose
Permeability
Carbon

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Hexose phosphorylation and the putative calcium channel component Mid1p are required for the hexose-induced transient elevation of cytosolic calcium response in Saccharomyces cerevisiae. / Tökés-Füzesi, Margit; Bedwell, David M.; Repa, I.; Sipos, Katalin; Sümegi, B.; Rab, András; Miseta, A.

In: Molecular Microbiology, Vol. 44, No. 5, 2002, p. 1299-1308.

Research output: Contribution to journalArticle

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