Hexavalent chromium uptake by sensitive and tolerant mutants of Schizosaccharomyces pombe

Klára Czakó-Vér, Martin Batiè, Peter Raspor, Mathias Sipiczki, Miklós Pesti

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Abstract

Lysine and leucine auxotrophic, heterothallic (h+, h-) strains of Schizosaccharomyces pombe were used to obtain chromium(VI)-sensitive and -tolerant mutants by ultraviolet radiation-induced and nitrosoguanidine-induced mutagenesis. The minimal inhibitory concentrations of K2Cr2O7 on YEA media were 225 μM for the wild-type strain CW-6, 125 μM for the sensitive mutant CS-6.51 and 275 μM for the tolerant mutant CT-6.66. The mutants exhibited cross-sensitivity of various patterns to Cd2+, Cu2+, Ni2+, Zn2+ and VO43-. Cr(VI) was added to the actively growing cultures and the total chromium (TOCr) content of the cells was determined. The sensitive mutant exhibited a high bioaccumulation ability, with a dry biomass of 810 μg g-1 after 30 min, while the tolerant mutant had a significantly lower ability than the wild-type strain. In PIPES buffer, washed, lysine-starved biomasses were treated with 75 μM Cr(VI) and after 2 h, the TOCr and the organically bound chromium (OBCr) were determined. Under these conditions, the sensitive and tolerant mutants had the same TOCr content, 50% of which was OBCr. The wild-type strain exhibited a lower TOCr content than that of its mutants and only 35% of this was OBCr. The Cr(VI)-sensitivity was due to a significantly increased uptake of Cr(VI). Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalFEMS microbiology letters
Volume178
Issue number1
DOIs
Publication statusPublished - Sep 1 1999

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Keywords

  • Cr(VI) uptake
  • Cr(VI)-sensitivity
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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