Heterotopic renal autotransplantation in a porcine model

A step-by-step protocol

J. Moritz Kaths, Juan Echeverri, Nicolas Goldaracena, Kristine S. Louis, Paul Yip, Rohan John, I. Mucsi, Anand Ghanekar, Darius Bagli, Markus Selzner, Lisa A. Robinson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Kidney transplantation is the treatment of choice for patients suffering from end-stage renal disease. It offers better life expectancy and higher quality of life when compared to dialysis. Although the last few decades have seen major improvements in patient outcomes following kidney transplantation, the increasing shortage of available organs represents a severe problem worldwide. To expand the donor pool, marginal kidney grafts recovered from extended criteria donors (ECD) or donated after circulatory death (DCD) are now accepted for transplantation. To further improve the postoperative outcome of these marginal grafts, research must focus on new therapeutic approaches such as alternative preservation techniques, immunomodulation, gene transfer, and stem cell administration. Experimental studies in animal models are the final step before newly developed techniques can be translated into clinical practice. Porcine kidney transplantation is an excellent model of human transplantation and allows investigation of novel approaches. The major advantage of the porcine model is its anatomical and physiological similarity to the human body, which facilitates the rapid translation of new findings to clinical trials. This article offers a surgical step-by-step protocol for an autotransplantation model and highlights key factors to ensure experimental success. Adequate pre- and postoperative housing, attentive anesthesia, and consistent surgical techniques result in favorable postoperative outcomes. Resection of the contralateral native kidney provides the opportunity to assess post-transplant graft function. The placement of venous and urinary catheters and the use of metabolic cages allow further detailed evaluation. For long-term follow-up studies and investigation of alternative graft preservation techniques, autotransplantation models are superior to allotransplantation models, as they avoid the confounding bias posed by rejection and immunosuppressive medication.

Original languageEnglish
Article numbere53765
JournalJournal of visualized experiments : JoVE
Volume2016
Issue number108
DOIs
Publication statusPublished - Feb 21 2016

Fingerprint

Autologous Transplantation
Swine
Transplants
Kidney
Grafts
Kidney Transplantation
Transplantation
Tissue Donors
Gene Transfer Techniques
Urinary Catheters
Immunomodulation
Gene transfer
Immunosuppressive Agents
Life Expectancy
Human Body
Dialysis
Catheters
Chronic Kidney Failure
Stem cells
Stem Cells

Keywords

  • Auto-allotransplantation
  • Delayed graft function (DGF)
  • Issue 108
  • Kidney
  • Marginal graft
  • Medicine
  • Organ preservation technique
  • Organ shortage
  • Organ transplantation
  • Pig
  • Porcine
  • Primary non-function (PNF)
  • Renal
  • Swine model

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Kaths, J. M., Echeverri, J., Goldaracena, N., Louis, K. S., Yip, P., John, R., ... Robinson, L. A. (2016). Heterotopic renal autotransplantation in a porcine model: A step-by-step protocol. Journal of visualized experiments : JoVE, 2016(108), [e53765]. https://doi.org/10.3791/53765

Heterotopic renal autotransplantation in a porcine model : A step-by-step protocol. / Kaths, J. Moritz; Echeverri, Juan; Goldaracena, Nicolas; Louis, Kristine S.; Yip, Paul; John, Rohan; Mucsi, I.; Ghanekar, Anand; Bagli, Darius; Selzner, Markus; Robinson, Lisa A.

In: Journal of visualized experiments : JoVE, Vol. 2016, No. 108, e53765, 21.02.2016.

Research output: Contribution to journalArticle

Kaths, JM, Echeverri, J, Goldaracena, N, Louis, KS, Yip, P, John, R, Mucsi, I, Ghanekar, A, Bagli, D, Selzner, M & Robinson, LA 2016, 'Heterotopic renal autotransplantation in a porcine model: A step-by-step protocol', Journal of visualized experiments : JoVE, vol. 2016, no. 108, e53765. https://doi.org/10.3791/53765
Kaths JM, Echeverri J, Goldaracena N, Louis KS, Yip P, John R et al. Heterotopic renal autotransplantation in a porcine model: A step-by-step protocol. Journal of visualized experiments : JoVE. 2016 Feb 21;2016(108). e53765. https://doi.org/10.3791/53765
Kaths, J. Moritz ; Echeverri, Juan ; Goldaracena, Nicolas ; Louis, Kristine S. ; Yip, Paul ; John, Rohan ; Mucsi, I. ; Ghanekar, Anand ; Bagli, Darius ; Selzner, Markus ; Robinson, Lisa A. / Heterotopic renal autotransplantation in a porcine model : A step-by-step protocol. In: Journal of visualized experiments : JoVE. 2016 ; Vol. 2016, No. 108.
@article{8b5f4379441b48a7b6f84a9f1eb02486,
title = "Heterotopic renal autotransplantation in a porcine model: A step-by-step protocol",
abstract = "Kidney transplantation is the treatment of choice for patients suffering from end-stage renal disease. It offers better life expectancy and higher quality of life when compared to dialysis. Although the last few decades have seen major improvements in patient outcomes following kidney transplantation, the increasing shortage of available organs represents a severe problem worldwide. To expand the donor pool, marginal kidney grafts recovered from extended criteria donors (ECD) or donated after circulatory death (DCD) are now accepted for transplantation. To further improve the postoperative outcome of these marginal grafts, research must focus on new therapeutic approaches such as alternative preservation techniques, immunomodulation, gene transfer, and stem cell administration. Experimental studies in animal models are the final step before newly developed techniques can be translated into clinical practice. Porcine kidney transplantation is an excellent model of human transplantation and allows investigation of novel approaches. The major advantage of the porcine model is its anatomical and physiological similarity to the human body, which facilitates the rapid translation of new findings to clinical trials. This article offers a surgical step-by-step protocol for an autotransplantation model and highlights key factors to ensure experimental success. Adequate pre- and postoperative housing, attentive anesthesia, and consistent surgical techniques result in favorable postoperative outcomes. Resection of the contralateral native kidney provides the opportunity to assess post-transplant graft function. The placement of venous and urinary catheters and the use of metabolic cages allow further detailed evaluation. For long-term follow-up studies and investigation of alternative graft preservation techniques, autotransplantation models are superior to allotransplantation models, as they avoid the confounding bias posed by rejection and immunosuppressive medication.",
keywords = "Auto-allotransplantation, Delayed graft function (DGF), Issue 108, Kidney, Marginal graft, Medicine, Organ preservation technique, Organ shortage, Organ transplantation, Pig, Porcine, Primary non-function (PNF), Renal, Swine model",
author = "Kaths, {J. Moritz} and Juan Echeverri and Nicolas Goldaracena and Louis, {Kristine S.} and Paul Yip and Rohan John and I. Mucsi and Anand Ghanekar and Darius Bagli and Markus Selzner and Robinson, {Lisa A.}",
year = "2016",
month = "2",
day = "21",
doi = "10.3791/53765",
language = "English",
volume = "2016",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "108",

}

TY - JOUR

T1 - Heterotopic renal autotransplantation in a porcine model

T2 - A step-by-step protocol

AU - Kaths, J. Moritz

AU - Echeverri, Juan

AU - Goldaracena, Nicolas

AU - Louis, Kristine S.

AU - Yip, Paul

AU - John, Rohan

AU - Mucsi, I.

AU - Ghanekar, Anand

AU - Bagli, Darius

AU - Selzner, Markus

AU - Robinson, Lisa A.

PY - 2016/2/21

Y1 - 2016/2/21

N2 - Kidney transplantation is the treatment of choice for patients suffering from end-stage renal disease. It offers better life expectancy and higher quality of life when compared to dialysis. Although the last few decades have seen major improvements in patient outcomes following kidney transplantation, the increasing shortage of available organs represents a severe problem worldwide. To expand the donor pool, marginal kidney grafts recovered from extended criteria donors (ECD) or donated after circulatory death (DCD) are now accepted for transplantation. To further improve the postoperative outcome of these marginal grafts, research must focus on new therapeutic approaches such as alternative preservation techniques, immunomodulation, gene transfer, and stem cell administration. Experimental studies in animal models are the final step before newly developed techniques can be translated into clinical practice. Porcine kidney transplantation is an excellent model of human transplantation and allows investigation of novel approaches. The major advantage of the porcine model is its anatomical and physiological similarity to the human body, which facilitates the rapid translation of new findings to clinical trials. This article offers a surgical step-by-step protocol for an autotransplantation model and highlights key factors to ensure experimental success. Adequate pre- and postoperative housing, attentive anesthesia, and consistent surgical techniques result in favorable postoperative outcomes. Resection of the contralateral native kidney provides the opportunity to assess post-transplant graft function. The placement of venous and urinary catheters and the use of metabolic cages allow further detailed evaluation. For long-term follow-up studies and investigation of alternative graft preservation techniques, autotransplantation models are superior to allotransplantation models, as they avoid the confounding bias posed by rejection and immunosuppressive medication.

AB - Kidney transplantation is the treatment of choice for patients suffering from end-stage renal disease. It offers better life expectancy and higher quality of life when compared to dialysis. Although the last few decades have seen major improvements in patient outcomes following kidney transplantation, the increasing shortage of available organs represents a severe problem worldwide. To expand the donor pool, marginal kidney grafts recovered from extended criteria donors (ECD) or donated after circulatory death (DCD) are now accepted for transplantation. To further improve the postoperative outcome of these marginal grafts, research must focus on new therapeutic approaches such as alternative preservation techniques, immunomodulation, gene transfer, and stem cell administration. Experimental studies in animal models are the final step before newly developed techniques can be translated into clinical practice. Porcine kidney transplantation is an excellent model of human transplantation and allows investigation of novel approaches. The major advantage of the porcine model is its anatomical and physiological similarity to the human body, which facilitates the rapid translation of new findings to clinical trials. This article offers a surgical step-by-step protocol for an autotransplantation model and highlights key factors to ensure experimental success. Adequate pre- and postoperative housing, attentive anesthesia, and consistent surgical techniques result in favorable postoperative outcomes. Resection of the contralateral native kidney provides the opportunity to assess post-transplant graft function. The placement of venous and urinary catheters and the use of metabolic cages allow further detailed evaluation. For long-term follow-up studies and investigation of alternative graft preservation techniques, autotransplantation models are superior to allotransplantation models, as they avoid the confounding bias posed by rejection and immunosuppressive medication.

KW - Auto-allotransplantation

KW - Delayed graft function (DGF)

KW - Issue 108

KW - Kidney

KW - Marginal graft

KW - Medicine

KW - Organ preservation technique

KW - Organ shortage

KW - Organ transplantation

KW - Pig

KW - Porcine

KW - Primary non-function (PNF)

KW - Renal

KW - Swine model

UR - http://www.scopus.com/inward/record.url?scp=84961275734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961275734&partnerID=8YFLogxK

U2 - 10.3791/53765

DO - 10.3791/53765

M3 - Article

VL - 2016

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 108

M1 - e53765

ER -