Heterologous expression of astaxanthin biosynthesis genes in Mucor circinelloides

Tamás Papp, Antonio Velayos, Tibor Bartók, Arturo P. Eslava, Csaba Vágvölgyi, Enrique A. Iturriaga

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Most Mucor species accumulate β-carotene as the main carotenoid. The crtW and crtZ astaxanthin biosynthesis genes from Agrobacterium aurantiacum were placed under the control of Mucor circinelloides expression signals. Expression vectors containing the bacterial genes were constructed, and PEG-mediated transformations were performed on a selected M. circinelloides strain. Transformants that exhibited altered carotene production were isolated and analyzed. Southern analysis showed that all plasmids behave as autoreplicative elements. Northern analysis detected the actual heterologous transcription products, whereas thin layer chromatography and high-performance liquid chromatography studies revealed the presence of new carotenoid compounds and intermediates among the transformants.

Original languageEnglish
Pages (from-to)526-531
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume69
Issue number5
DOIs
Publication statusPublished - Jan 1 2006

    Fingerprint

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this