Heterologous expression and protein engineering of wheat gluten proteins

L. Tamás, Peter R. Shewry

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A range of systems are available for the production of recombinant wheat gluten proteins, from simple and widely used systems based on Escherichia coli to more sophisticated eukaryotic systems in yeasts or cultured insect cells. The characteristics of these systems are summarised and their advantages and disadvantages for application to wheat gluten proteins discussed. We then review the applications of heterologous expression systems to the synthesis and characterisation of wheat gluten proteins, including the production of wild type and mutant proteins for structure-function studies. We also discuss the use of heterologous expression to establish model systems including perfect repeat peptides based on motifs present in gliadins and glutenin subunits and 'analogue glutenin proteins' based on C hordein of barley. It is concluded that the pET series of vectors and E. coli are suitable for most applications, providing high-level expression and being rapid and easy to use.

Original languageEnglish
Pages (from-to)259-274
Number of pages16
JournalJournal of Cereal Science
Volume43
Issue number3
DOIs
Publication statusPublished - May 2006

Fingerprint

protein engineering
Protein Engineering
wheat gluten
Glutens
Triticum
glutenins
Escherichia coli
Proteins
proteins
hordein
Gliadin
gliadin
Mutant Proteins
Hordeum
protein structure
Yeast
Insects
Cultured Cells
Yeasts
barley

Keywords

  • E. coli
  • Protein engineering
  • Recombinant proteins
  • Wheat gluten proteins

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science

Cite this

Heterologous expression and protein engineering of wheat gluten proteins. / Tamás, L.; Shewry, Peter R.

In: Journal of Cereal Science, Vol. 43, No. 3, 05.2006, p. 259-274.

Research output: Contribution to journalArticle

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