Bovine adrenal cortical microsomes possess high (K(d) = 3.68 ± 1.02 x 10-9 M) and lower (K(d) = 9.20 ± 1.71 x 10-8 M) affinity binding sites for inositol 1,3,4,5-tetrakisphosphate. The binding to these sites is rapid, saturable (reaches equilibrium by 15 min at 0°C), and reversible. Competition studies with other inositol phosphate analogs indicate that the high affinity binding sites are clearly distinct from the inositol 1,4,5-trisphosphate receptors which are, however, responsible for a fraction of the lower affinity binding. The characteristics of the inositol 1,3,4,5-tetrakisphosphate binding sites described are compatible with their possible receptor function.
|Number of pages||3|
|Journal||Journal of Biological Chemistry|
|Publication status||Published - Jan 1 1988|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology