Heterogeneity of chicken photoreceptors as defined by hybridoma supernatants - An immunocytochemical study

A. Szél, L. Takács, E. Monostori, I. Vigh-Teichmann, P. Röhlich

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Immune cells producing antibodies to chicken photoreceptor membranes were fused with myeloma cells and supernatants of the resulting hybridoma cells were used to test various types of photoreceptor cells in the chicken retina by means of immunocytochemistry. A polyclonal antibody raised against the protein component of bovine rhodopsin was also used. Outer segments of various photoreceptor cells were labelled by the following antibodies: rods were positive with the anti-rhodopsin antibody, both members of the double cones were stained by supernatant A1, while one type of single cones (designated as type A) was specifically labelled by supernatants A5, B3 and D6. The other type of single cones (type B) reacted with anti-rhodopsin and supernatant A1. The results indicate that there are distinct differences in the molecular structure of various photoreceptor outer segments.

Original languageEnglish
Pages (from-to)735-741
Number of pages7
JournalCell and Tissue Research
Volume240
Issue number3
DOIs
Publication statusPublished - Jun 1985

Fingerprint

Rhodopsin
Hybridomas
Chickens
Photoreceptor Cells
Cones
Antibodies
Antibody-Producing Cells
Molecular Structure
Retina
Anti-Idiotypic Antibodies
Immunohistochemistry
Molecular structure
Membranes
Proteins

Keywords

  • Antibodies
  • Immunocytochemistry
  • Immunological heterogeneity
  • Photopigments
  • Photoreceptor outer segments
  • Retina, chicken

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Heterogeneity of chicken photoreceptors as defined by hybridoma supernatants - An immunocytochemical study. / Szél, A.; Takács, L.; Monostori, E.; Vigh-Teichmann, I.; Röhlich, P.

In: Cell and Tissue Research, Vol. 240, No. 3, 06.1985, p. 735-741.

Research output: Contribution to journalArticle

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