Herpes simplex virus decreases endothelial cell plasminogen activator inhibitor

R. A. Bok, H. S. Jacob, J. Balla, M. Juckett, T. Stella, M. A. Shatos, G. M. Vercellotti

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Herpes simplex virus (HSV) infection is histopathologically associated with vascular injury, fibrinoid necrosis and inflammatory cell infiltrates. We have previously shown in vitro that HSV infection of human umbilical vein endothelial cells (HUVEC) promotes a procoagulant phenotype manifest by the induction of tissue factor, the loss of thrombomodulin, and an increase in platelet adhesion. In these studies we examined the effects of HSV infection on HUVEC plasminogen activator inhibitor type 1 (PAI-1) and tissue plasminogen activator (t-PA). HSV infection caused the loss of PAI-1 in the extracellular matrix (ECM) and that released into the supernatant of HUVEC. Both activity and antigen levels of the Serpin inhibitor are diminished as a result of HSV infection. The loss of inhibitor is not secondary to diminished vitronectin (Vn), the primary binding protein of PAI-1 in the ECM, but appears to be secondary to decreased synthesis at the RNA level. Tissue plasminogen activator (t-PA) synthesis is also decreased in endothelial HSV infection. PAI-1 loss may further promote a procoagulant phenotype in HSV infection in vivo.

Original languageEnglish
Pages (from-to)253-258
Number of pages6
JournalThrombosis and Haemostasis
Volume69
Issue number3
Publication statusPublished - 1993

Fingerprint

Plasminogen Inactivators
Virus Diseases
Simplexvirus
Endothelial Cells
Plasminogen Activator Inhibitor 1
Human Umbilical Vein Endothelial Cells
Tissue Plasminogen Activator
Extracellular Matrix
Serpins
Phenotype
Vitronectin
Thrombomodulin
Vascular System Injuries
Thromboplastin
Carrier Proteins
Necrosis
Blood Platelets
RNA
Antigens

ASJC Scopus subject areas

  • Hematology

Cite this

Bok, R. A., Jacob, H. S., Balla, J., Juckett, M., Stella, T., Shatos, M. A., & Vercellotti, G. M. (1993). Herpes simplex virus decreases endothelial cell plasminogen activator inhibitor. Thrombosis and Haemostasis, 69(3), 253-258.

Herpes simplex virus decreases endothelial cell plasminogen activator inhibitor. / Bok, R. A.; Jacob, H. S.; Balla, J.; Juckett, M.; Stella, T.; Shatos, M. A.; Vercellotti, G. M.

In: Thrombosis and Haemostasis, Vol. 69, No. 3, 1993, p. 253-258.

Research output: Contribution to journalArticle

Bok, RA, Jacob, HS, Balla, J, Juckett, M, Stella, T, Shatos, MA & Vercellotti, GM 1993, 'Herpes simplex virus decreases endothelial cell plasminogen activator inhibitor', Thrombosis and Haemostasis, vol. 69, no. 3, pp. 253-258.
Bok RA, Jacob HS, Balla J, Juckett M, Stella T, Shatos MA et al. Herpes simplex virus decreases endothelial cell plasminogen activator inhibitor. Thrombosis and Haemostasis. 1993;69(3):253-258.
Bok, R. A. ; Jacob, H. S. ; Balla, J. ; Juckett, M. ; Stella, T. ; Shatos, M. A. ; Vercellotti, G. M. / Herpes simplex virus decreases endothelial cell plasminogen activator inhibitor. In: Thrombosis and Haemostasis. 1993 ; Vol. 69, No. 3. pp. 253-258.
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