Hephaestia caeni gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from activated sludge

Tamás Felföldi, Anita Vengring, Károly Márialigeti, Judit András, Peter Schumann, Erika M. Tóth

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A Gram-staining-negative, rod-shaped and motile bacterium, designated strain ERB1-3T, was isolated from a laboratory-scale activated sludge system treating coke plant effluent using thiocyanate-supplemented growth medium. Strain ERB1-3T was oxidase-positive and weakly catalase-positive. The predominant fatty acids were C18: 1ω7c (35.6 %) and C17: 1ω6c (29.2 %), and the major respiratory quinone was Q-10. Polar lipids were dominated by sphingoglycolipid and phosphatidylglycerol. Major polyamines were spermidine and sym-homospermidine. The G+C content of the genomic DNA of strain ERB1-3T was 66.4 mol%. Based on the 16S rRNA gene, strain ERB1-3T exhibited the highest sequence similarity values to Sphingomonas sanxanigenens DSM 19645T (96.1 %), Sphingobium scionense DSM 19371T(95.1 %) and Stakelama pacifica LMG 24686T (94.8 %) within the familySphingomonadaceae. The novel isolate had some unique chemotaxonomic features that differentiated it from these closely related strains, contained much more C17: 1ω6c, C15: 0 2-OH, C17: 0 and C17: 1ω8c fatty acids and possessed diphosphatidylglycerol only in trace amounts. On the basis of the phenotypic, chemotaxonomic and molecular data, strain ERB1-3T is considered to represent a novel genus and species, for which the nameHephaestia caeni gen. nov., sp. nov. is proposed. The type strain is ERB1-3T(= DSM 25527T = NCAIM B 02511T).

Original languageEnglish
Pages (from-to)738-744
Number of pages7
JournalInternational journal of systematic and evolutionary microbiology
Issue numberPART 3
Publication statusPublished - Mar 17 2014


ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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