Hepatoprotective effects of a self-micro emulsifying drug delivery system containing Silybum marianum native seed oil against experimentally induced liver injury

P. Fehér, Z. Ujhelyi, M. Vecsernyés, F. Fenyvesi, G. Damache, A. Ardelean, M. Costache, A. Dinischiotu, A. Hermenean, I. Bácskay

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The main purpose of this study was to certify the effect of native silymarin oil (SM-oil) formulated in a self-microemulsifying drug delivery system (SMEDDS). The optimal formulation was 25 % of SM-oil, 33,3 % of Cremophor RH40, 20 % of Transcutol HP, 16,6 % of Labrasol and 5 % of Capryol 90. In this novel formulation the SM-oil was the active substance and the lipid part. The in vivo study examined the preventive effects of SMEDDS containing SM native seeds oil against carbon tetrachloride (CCl4) induced hepatotoxicity in mice. Determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST) levels and also liver histology investigations have been done. The liver antioxidant status was determined with the concentrations of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione (GSH) hepatic lipid peroxidation was examined and expressed in terms of malondialdehyde (MDA) content. The plasma levels of AST and ALT significantly diminished by pretreatment with 500 mg/kg and 1000 mg/kg SMEDDS. The pre-treatment with 500 mg/kg and 1000 mg/kg SMEDDS increased GSH level by about 6% respectively 24% compared to the CCl4 group. Due to preventive administration of 500 mg/kg and 1000 mg/kg of SMEDDS in the intoxicated animals, MDA levels were reduced by 22% respectively 58%. Also, an insignificant rise by almost 17% and 19% in the animals treated with the both doses of SMEDDS could be noticed. It can be concluded that hepatotoxicity may be voided by the oral application of our formulation.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalPharmazie
Volume70
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

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Milk Thistle
Drug Delivery Systems
Seeds
Oils
Silymarin
Liver
Wounds and Injuries
Aspartate Aminotransferases
Malondialdehyde
Alanine Transaminase
Glutathione Reductase
Carbon Tetrachloride
Glutathione Peroxidase
Catalase
Lipid Peroxidation
Superoxide Dismutase
Glutathione
Histology
Antioxidants
Lipids

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Medicine(all)

Cite this

Hepatoprotective effects of a self-micro emulsifying drug delivery system containing Silybum marianum native seed oil against experimentally induced liver injury. / Fehér, P.; Ujhelyi, Z.; Vecsernyés, M.; Fenyvesi, F.; Damache, G.; Ardelean, A.; Costache, M.; Dinischiotu, A.; Hermenean, A.; Bácskay, I.

In: Pharmazie, Vol. 70, No. 4, 01.04.2015, p. 231-238.

Research output: Contribution to journalArticle

Fehér, P. ; Ujhelyi, Z. ; Vecsernyés, M. ; Fenyvesi, F. ; Damache, G. ; Ardelean, A. ; Costache, M. ; Dinischiotu, A. ; Hermenean, A. ; Bácskay, I. / Hepatoprotective effects of a self-micro emulsifying drug delivery system containing Silybum marianum native seed oil against experimentally induced liver injury. In: Pharmazie. 2015 ; Vol. 70, No. 4. pp. 231-238.
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AU - Damache, G.

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AU - Bácskay, I.

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