Hepatocyte proliferation and cell cycle phase fractions in chronic viral hepatitis C by image analysis method

Klara Werling, Zoltán Szentirmay, Ágota Szepesi, Zsuzsa Schaff, Ferenc Szalay, Zsuzsa Szabó, László Telegdy, Károly Dávid, Gyula Stotz, Zsolt Tulassay

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective Chronic hepatitis is characterized by necrosis of liver cells, accompanied by an inflammatory reaction and compensatory cell proliferation. The interaction of the core and non-structural proteins of hepatitis C virus (HCV) with several cellular factors suggests that cell proliferation may be influenced by HCV. The aim of this study was to investigate hepatocyte proliferation and DNA ploidy patterns in patients with chronic viral hepatitis C (CH-C) compared with chronic non-viral hepatitis (CH-N), using a TV image analysis method. Methods The DNA index (DI) and cell phase fractions (G1, S, G2) were measured by means of digital picture analysis method on nuclear suspensions of Feulgen stained hepatocytes. Cells were taken from the liver biopsy specimens of 71 patients with CH-C and 24 patients with CH-N. Twenty-six normal liver samples were used as controls. Results Significantly higher G1 (94 ± 4) and lower S (3.56 ± 3.16) phase fractions were measured in CH-C compared with CH-N (G1, 90 ± 6; S, 6.4 ± 5.99). The DI of moderate (1.12 ± 0.05) and severe (1.12 ± 0.05) CH-C showed near-aneuploid DNA content, while diploidy (DI < 1.10) was detected in cases of CH-N. Conclusion The higher G1 and lower S cell cycle phase fractions in CH-C reflect decreased hepatocyte proliferation compared with CH-N. The near-aneuploid DNA content of the HCV-infected liver samples may be a sign of increased genetic instability, which may contribute to the carcinogenic potential of HCV.

Original languageEnglish
Pages (from-to)489-493
Number of pages5
JournalEuropean Journal of Gastroenterology and Hepatology
Volume13
Issue number5
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Cell cycle phase fractions
  • Chronic viral hepatitis C
  • DNA ploidy
  • Hepatocyte proliferation
  • Image analysis

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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