Hepatocyte proliferation and cell cycle phase fractions in chronic viral hepatitis C by image analysis method

Klara Werling, Z. Szentirmay, Ágota Szepesi, Z. Schaff, F. Szalay, Zsuzsa Szabó, L. Telegdy, Károly Dávid, Gyula Stotz, Z. Tulassay

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: Chronic hepatitis is characterized by necrosis of liver cells, accompanied by an inflammatory reaction and compensatory cell proliferation. The interaction of the core and non-structural proteins of hepatitis C virus (HCV) with several cellular factors suggests that cell proliferation may be influenced by HCV. The aim of this study was to investigate hepatocyte proliferation and DNA ploidy patterns in patients with chronic viral hepatitis C (CH-C) compared with chronic non-viral hepatitis (CH-N), using a TV image analysis method. Methods: The DNA index (DI) and cell phase fractions (G1, S, G2) were measured by means of digital picture analysis method on nuclear suspensions of Feulgen stained hepatocytes. Cells were taken from the liver biopsy specimens of 71 patients with CH-C and 24 patients with CH-N. Twenty-six normal liver samples were used as controls. Results: Significantly higher G1 (94 ± 4) and lower S (3.56 ± 3.16) phase fractions were measured in CH-C compared with CH-N (G1, 90 ± 6; S, 6.4 ± 5.99). The DI of moderate (1.12 ± 0.05) and severe (1.12 ± 0.05) CH-C showed near-aneuploid DNA content, while diploidy (DI <1.10) was detected in cases of CH-N. Conclusion: The higher G1 and lower S cell cycle phase fractions in CH-C reflect decreased hepatocyte proliferation compared with CH-N. The near-aneuploid DNA content of the HCV-infected liver samples may be a sign of increased genetic instability, which may contribute to the carcinogenic potential of HCV.

Original languageEnglish
Pages (from-to)489-493
Number of pages5
JournalEuropean Journal of Gastroenterology and Hepatology
Volume13
Issue number5
DOIs
Publication statusPublished - 2001

Fingerprint

Chronic Hepatitis C
Hepatocytes
Cell Cycle
Hepacivirus
DNA
Liver
Aneuploidy
Hepatitis
Cell Proliferation
Ploidies
G1 Phase
Chronic Hepatitis
Protein C
Diploidy
Suspensions
Necrosis
Biopsy

Keywords

  • Cell cycle phase fractions
  • Chronic viral hepatitis C
  • DNA ploidy
  • Hepatocyte proliferation
  • Image analysis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Hepatocyte proliferation and cell cycle phase fractions in chronic viral hepatitis C by image analysis method. / Werling, Klara; Szentirmay, Z.; Szepesi, Ágota; Schaff, Z.; Szalay, F.; Szabó, Zsuzsa; Telegdy, L.; Dávid, Károly; Stotz, Gyula; Tulassay, Z.

In: European Journal of Gastroenterology and Hepatology, Vol. 13, No. 5, 2001, p. 489-493.

Research output: Contribution to journalArticle

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abstract = "Objective: Chronic hepatitis is characterized by necrosis of liver cells, accompanied by an inflammatory reaction and compensatory cell proliferation. The interaction of the core and non-structural proteins of hepatitis C virus (HCV) with several cellular factors suggests that cell proliferation may be influenced by HCV. The aim of this study was to investigate hepatocyte proliferation and DNA ploidy patterns in patients with chronic viral hepatitis C (CH-C) compared with chronic non-viral hepatitis (CH-N), using a TV image analysis method. Methods: The DNA index (DI) and cell phase fractions (G1, S, G2) were measured by means of digital picture analysis method on nuclear suspensions of Feulgen stained hepatocytes. Cells were taken from the liver biopsy specimens of 71 patients with CH-C and 24 patients with CH-N. Twenty-six normal liver samples were used as controls. Results: Significantly higher G1 (94 ± 4) and lower S (3.56 ± 3.16) phase fractions were measured in CH-C compared with CH-N (G1, 90 ± 6; S, 6.4 ± 5.99). The DI of moderate (1.12 ± 0.05) and severe (1.12 ± 0.05) CH-C showed near-aneuploid DNA content, while diploidy (DI <1.10) was detected in cases of CH-N. Conclusion: The higher G1 and lower S cell cycle phase fractions in CH-C reflect decreased hepatocyte proliferation compared with CH-N. The near-aneuploid DNA content of the HCV-infected liver samples may be a sign of increased genetic instability, which may contribute to the carcinogenic potential of HCV.",
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AU - Werling, Klara

AU - Szentirmay, Z.

AU - Szepesi, Ágota

AU - Schaff, Z.

AU - Szalay, F.

AU - Szabó, Zsuzsa

AU - Telegdy, L.

AU - Dávid, Károly

AU - Stotz, Gyula

AU - Tulassay, Z.

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N2 - Objective: Chronic hepatitis is characterized by necrosis of liver cells, accompanied by an inflammatory reaction and compensatory cell proliferation. The interaction of the core and non-structural proteins of hepatitis C virus (HCV) with several cellular factors suggests that cell proliferation may be influenced by HCV. The aim of this study was to investigate hepatocyte proliferation and DNA ploidy patterns in patients with chronic viral hepatitis C (CH-C) compared with chronic non-viral hepatitis (CH-N), using a TV image analysis method. Methods: The DNA index (DI) and cell phase fractions (G1, S, G2) were measured by means of digital picture analysis method on nuclear suspensions of Feulgen stained hepatocytes. Cells were taken from the liver biopsy specimens of 71 patients with CH-C and 24 patients with CH-N. Twenty-six normal liver samples were used as controls. Results: Significantly higher G1 (94 ± 4) and lower S (3.56 ± 3.16) phase fractions were measured in CH-C compared with CH-N (G1, 90 ± 6; S, 6.4 ± 5.99). The DI of moderate (1.12 ± 0.05) and severe (1.12 ± 0.05) CH-C showed near-aneuploid DNA content, while diploidy (DI <1.10) was detected in cases of CH-N. Conclusion: The higher G1 and lower S cell cycle phase fractions in CH-C reflect decreased hepatocyte proliferation compared with CH-N. The near-aneuploid DNA content of the HCV-infected liver samples may be a sign of increased genetic instability, which may contribute to the carcinogenic potential of HCV.

AB - Objective: Chronic hepatitis is characterized by necrosis of liver cells, accompanied by an inflammatory reaction and compensatory cell proliferation. The interaction of the core and non-structural proteins of hepatitis C virus (HCV) with several cellular factors suggests that cell proliferation may be influenced by HCV. The aim of this study was to investigate hepatocyte proliferation and DNA ploidy patterns in patients with chronic viral hepatitis C (CH-C) compared with chronic non-viral hepatitis (CH-N), using a TV image analysis method. Methods: The DNA index (DI) and cell phase fractions (G1, S, G2) were measured by means of digital picture analysis method on nuclear suspensions of Feulgen stained hepatocytes. Cells were taken from the liver biopsy specimens of 71 patients with CH-C and 24 patients with CH-N. Twenty-six normal liver samples were used as controls. Results: Significantly higher G1 (94 ± 4) and lower S (3.56 ± 3.16) phase fractions were measured in CH-C compared with CH-N (G1, 90 ± 6; S, 6.4 ± 5.99). The DI of moderate (1.12 ± 0.05) and severe (1.12 ± 0.05) CH-C showed near-aneuploid DNA content, while diploidy (DI <1.10) was detected in cases of CH-N. Conclusion: The higher G1 and lower S cell cycle phase fractions in CH-C reflect decreased hepatocyte proliferation compared with CH-N. The near-aneuploid DNA content of the HCV-infected liver samples may be a sign of increased genetic instability, which may contribute to the carcinogenic potential of HCV.

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