Hepatic lesions in Lebistes reticulatus induced by N-nitrosodiethylamine.

K. Simon, L. Tajti, K. Lapis

Research output: Contribution to journalArticle

Abstract

The effect of N-nitrosodiethylamine (NDEA) on the aquarium fish, Lebistes reticulatus (guppy) has been investigated. The aquarium water contained 10 to 200 parts per million of NDEA. The LC50/30 for NDEA was 100 ppm. Sub-chronic experiments were carried out using 25 and 50 ppm for 90 days. The liver alterations were investigated morphologically. Initially, diffuse necrosis of hepatic cells, disorientation of hepatic structure and cyst-formation appeared. The degenerative lesions were followed by multiple foci of regenerating hepatic cells. In the later period of the experiment, hyperplastic nodules of hepatocytes, cholangiofibrosis, cystic cholangiomas, hepatocellular and cholangiocellular carcinomas could be observed in the treated animals. The following conclusions have been reached: 1. Aquarium fish can be used as a model for short-term, in vivo carcinogenesis experiments. 2. Lebistes reticulatus is a suitable strain for use as a positive control with NDEA. 3. With this model, the process of carcinogenesis can be followed histologically. 4. The process is irreversible and some of its features may be used as an early indicator in the screening of possible carcinogenic substances.

Original languageEnglish
Pages (from-to)717-729
Number of pages13
JournalIARC scientific publications
Issue number31
Publication statusPublished - 1980

Fingerprint

Poecilia
Diethylnitrosamine
Hepatocytes
Liver
Bile Duct Adenoma
Carcinogenesis
Fishes
Cholangiocarcinoma
Cysts
Hepatocellular Carcinoma
Necrosis
Water

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hepatic lesions in Lebistes reticulatus induced by N-nitrosodiethylamine. / Simon, K.; Tajti, L.; Lapis, K.

In: IARC scientific publications, No. 31, 1980, p. 717-729.

Research output: Contribution to journalArticle

@article{7138571dd51b42f49fac05020f3a84ac,
title = "Hepatic lesions in Lebistes reticulatus induced by N-nitrosodiethylamine.",
abstract = "The effect of N-nitrosodiethylamine (NDEA) on the aquarium fish, Lebistes reticulatus (guppy) has been investigated. The aquarium water contained 10 to 200 parts per million of NDEA. The LC50/30 for NDEA was 100 ppm. Sub-chronic experiments were carried out using 25 and 50 ppm for 90 days. The liver alterations were investigated morphologically. Initially, diffuse necrosis of hepatic cells, disorientation of hepatic structure and cyst-formation appeared. The degenerative lesions were followed by multiple foci of regenerating hepatic cells. In the later period of the experiment, hyperplastic nodules of hepatocytes, cholangiofibrosis, cystic cholangiomas, hepatocellular and cholangiocellular carcinomas could be observed in the treated animals. The following conclusions have been reached: 1. Aquarium fish can be used as a model for short-term, in vivo carcinogenesis experiments. 2. Lebistes reticulatus is a suitable strain for use as a positive control with NDEA. 3. With this model, the process of carcinogenesis can be followed histologically. 4. The process is irreversible and some of its features may be used as an early indicator in the screening of possible carcinogenic substances.",
author = "K. Simon and L. Tajti and K. Lapis",
year = "1980",
language = "English",
pages = "717--729",
journal = "IARC (International Agency for Research on Cancer) Scientific Publications",
issn = "0300-5038",
publisher = "IARC",
number = "31",

}

TY - JOUR

T1 - Hepatic lesions in Lebistes reticulatus induced by N-nitrosodiethylamine.

AU - Simon, K.

AU - Tajti, L.

AU - Lapis, K.

PY - 1980

Y1 - 1980

N2 - The effect of N-nitrosodiethylamine (NDEA) on the aquarium fish, Lebistes reticulatus (guppy) has been investigated. The aquarium water contained 10 to 200 parts per million of NDEA. The LC50/30 for NDEA was 100 ppm. Sub-chronic experiments were carried out using 25 and 50 ppm for 90 days. The liver alterations were investigated morphologically. Initially, diffuse necrosis of hepatic cells, disorientation of hepatic structure and cyst-formation appeared. The degenerative lesions were followed by multiple foci of regenerating hepatic cells. In the later period of the experiment, hyperplastic nodules of hepatocytes, cholangiofibrosis, cystic cholangiomas, hepatocellular and cholangiocellular carcinomas could be observed in the treated animals. The following conclusions have been reached: 1. Aquarium fish can be used as a model for short-term, in vivo carcinogenesis experiments. 2. Lebistes reticulatus is a suitable strain for use as a positive control with NDEA. 3. With this model, the process of carcinogenesis can be followed histologically. 4. The process is irreversible and some of its features may be used as an early indicator in the screening of possible carcinogenic substances.

AB - The effect of N-nitrosodiethylamine (NDEA) on the aquarium fish, Lebistes reticulatus (guppy) has been investigated. The aquarium water contained 10 to 200 parts per million of NDEA. The LC50/30 for NDEA was 100 ppm. Sub-chronic experiments were carried out using 25 and 50 ppm for 90 days. The liver alterations were investigated morphologically. Initially, diffuse necrosis of hepatic cells, disorientation of hepatic structure and cyst-formation appeared. The degenerative lesions were followed by multiple foci of regenerating hepatic cells. In the later period of the experiment, hyperplastic nodules of hepatocytes, cholangiofibrosis, cystic cholangiomas, hepatocellular and cholangiocellular carcinomas could be observed in the treated animals. The following conclusions have been reached: 1. Aquarium fish can be used as a model for short-term, in vivo carcinogenesis experiments. 2. Lebistes reticulatus is a suitable strain for use as a positive control with NDEA. 3. With this model, the process of carcinogenesis can be followed histologically. 4. The process is irreversible and some of its features may be used as an early indicator in the screening of possible carcinogenic substances.

UR - http://www.scopus.com/inward/record.url?scp=0019288664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019288664&partnerID=8YFLogxK

M3 - Article

C2 - 7228293

AN - SCOPUS:0019288664

SP - 717

EP - 729

JO - IARC (International Agency for Research on Cancer) Scientific Publications

JF - IARC (International Agency for Research on Cancer) Scientific Publications

SN - 0300-5038

IS - 31

ER -