Heparin can liberate high molecular weight DNA from secondary necrotic cells

Research output: Contribution to journalArticle

Abstract

The borderline between necrosis and apoptosis is indistinct, but that between types of cell death is important because necrosis may lead to local inflammation, whereas apoptosis usually does not. In certain autoimmune disorders, inhibition of cell death is crucial, since macromolecules released from the dead cells may accelerate the autoimmune processes. We have used various cell death inhibitors to block cell death induced by 4HPR [N-(4-hydroxyphenil)-retinamide] the BL41 and U937 cell lines. VD-FMK, a general caspase inhibitor, inhibited DNA fragmentation induced by 4HPR, but not PI (propidium iodide) uptake and necrosis. Interestingly heparin, a serine-protease inhibitor, lowered the PI fluorescence of the dead cell population and increased the sub-G1 population as measured by flow cytometry. Regarding these changes, we found that heparin failed to increase DNA fragmentation, but merely liberated high molecular mass DNA fragments from dead cells. The exact mechanism is unclear, but heparin during secondary necrosis might enter the cells, bind RNPs (ribonucleoproteins), and pull them out with the attached DNA, where they would be sensitive to enzymatic degradation. Thus, the results suggest that heparin treatment helps in the clearance of cell debris and decreases the immunogenity of secondary necrotic cells.

Original languageEnglish
Pages (from-to)1281-1286
Number of pages6
JournalCell Biology International
Volume36
Issue number12
DOIs
Publication statusPublished - Dec 1 2012

Fingerprint

Heparin
Molecular Weight
DNA
Cell Death
Necrosis
Propidium
DNA Fragmentation
Apoptosis
U937 Cells
Serine Proteinase Inhibitors
Ribonucleoproteins
Caspase Inhibitors
Population
Flow Cytometry
Fluorescence
Inflammation
Cell Line
retinamide

Keywords

  • 4HPR
  • Apoptosis
  • Autoimmune disease
  • Heparin
  • Secondary necrosis
  • Z-VD-FMK

ASJC Scopus subject areas

  • Cell Biology

Cite this

Heparin can liberate high molecular weight DNA from secondary necrotic cells. / Barna, G.; Sebestyén, A.; Dunai, Zsuzsanna A.; Csernus, B.; Mihalik, R.

In: Cell Biology International, Vol. 36, No. 12, 01.12.2012, p. 1281-1286.

Research output: Contribution to journalArticle

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