Heparan sulfate proteoglycan of human colon: Partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1

George R. Dodge, I. Kovalszky, Mon Li Chu, John R. Hassell, O. Wesley McBride, Hua Fang Yi, Renato V. Iozzo

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We have determined the sequence of two overlapping cDNA clones encoding a portion of the human heparan sulfate proteoglycan core protein (HSPG2) from a human colon library. The cDNA clones encompassed 1.34 kb of nucleotide sequence and showed ∼85% homology to the murine heparan sulfate proteoglycan of the EHS tumor, BPG-5. The deduced amino acid sequence revealed an identity of 87% between the human and the murine species. Of the 57 different amino acids encoded in the human clones, 20 were substituted with a similar amino acid. Among the specific features that were highly conserved was the 32 cysteine residues with a distinctive repeat pattern characteristic of epidermal growth factor. Northern blotting revealed a single, ∼12 kb transcript in a variety of cells including human colon carcinoma, endothelial, and fibroblastic cells. The size of this transcript correlated with the estimated molecular weight (∼400 kDa) of the protein core precursor. Southern blot analyses of DNA from 120 human/rodent somatic cell hybrids, including subclones with specific translocations or spontaneous breaks of human chromosome 1, demonstrated the presence of HSPG2 gene on the telomeric region of the short arm of chromosome 1 (1p34-pter). Two polymorphisms with TaqI and EcoRI restriction endonucleases, respectively, were also detected. The localization of this proteoglycan locus in the human genome and the availability of new RFLPs provide the tools for future studies of human diseases where the HSPG2 proteoglycan gene is suspected to be involved.

Original languageEnglish
Pages (from-to)673-680
Number of pages8
JournalGenomics
Volume10
Issue number3
DOIs
Publication statusPublished - 1991

Fingerprint

Heparan Sulfate Proteoglycans
Chromosome Mapping
Chromosomes, Human, Pair 1
Human Chromosomes
Molecular Cloning
Colon
Gene Expression
Clone Cells
Proteoglycans
Experimental Sarcomas
Complementary DNA
Deoxyribonuclease EcoRI
Amino Acids
Protein Precursors
Hybrid Cells
DNA Restriction Enzymes
Human Genome
Southern Blotting
Epidermal Growth Factor
Restriction Fragment Length Polymorphisms

ASJC Scopus subject areas

  • Genetics

Cite this

Heparan sulfate proteoglycan of human colon : Partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1. / Dodge, George R.; Kovalszky, I.; Chu, Mon Li; Hassell, John R.; McBride, O. Wesley; Yi, Hua Fang; Iozzo, Renato V.

In: Genomics, Vol. 10, No. 3, 1991, p. 673-680.

Research output: Contribution to journalArticle

Dodge, George R. ; Kovalszky, I. ; Chu, Mon Li ; Hassell, John R. ; McBride, O. Wesley ; Yi, Hua Fang ; Iozzo, Renato V. / Heparan sulfate proteoglycan of human colon : Partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1. In: Genomics. 1991 ; Vol. 10, No. 3. pp. 673-680.
@article{5d118846f7864cc78b876c506e894c3c,
title = "Heparan sulfate proteoglycan of human colon: Partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1",
abstract = "We have determined the sequence of two overlapping cDNA clones encoding a portion of the human heparan sulfate proteoglycan core protein (HSPG2) from a human colon library. The cDNA clones encompassed 1.34 kb of nucleotide sequence and showed ∼85{\%} homology to the murine heparan sulfate proteoglycan of the EHS tumor, BPG-5. The deduced amino acid sequence revealed an identity of 87{\%} between the human and the murine species. Of the 57 different amino acids encoded in the human clones, 20 were substituted with a similar amino acid. Among the specific features that were highly conserved was the 32 cysteine residues with a distinctive repeat pattern characteristic of epidermal growth factor. Northern blotting revealed a single, ∼12 kb transcript in a variety of cells including human colon carcinoma, endothelial, and fibroblastic cells. The size of this transcript correlated with the estimated molecular weight (∼400 kDa) of the protein core precursor. Southern blot analyses of DNA from 120 human/rodent somatic cell hybrids, including subclones with specific translocations or spontaneous breaks of human chromosome 1, demonstrated the presence of HSPG2 gene on the telomeric region of the short arm of chromosome 1 (1p34-pter). Two polymorphisms with TaqI and EcoRI restriction endonucleases, respectively, were also detected. The localization of this proteoglycan locus in the human genome and the availability of new RFLPs provide the tools for future studies of human diseases where the HSPG2 proteoglycan gene is suspected to be involved.",
author = "Dodge, {George R.} and I. Kovalszky and Chu, {Mon Li} and Hassell, {John R.} and McBride, {O. Wesley} and Yi, {Hua Fang} and Iozzo, {Renato V.}",
year = "1991",
doi = "10.1016/0888-7543(91)90451-J",
language = "English",
volume = "10",
pages = "673--680",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Heparan sulfate proteoglycan of human colon

T2 - Partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1

AU - Dodge, George R.

AU - Kovalszky, I.

AU - Chu, Mon Li

AU - Hassell, John R.

AU - McBride, O. Wesley

AU - Yi, Hua Fang

AU - Iozzo, Renato V.

PY - 1991

Y1 - 1991

N2 - We have determined the sequence of two overlapping cDNA clones encoding a portion of the human heparan sulfate proteoglycan core protein (HSPG2) from a human colon library. The cDNA clones encompassed 1.34 kb of nucleotide sequence and showed ∼85% homology to the murine heparan sulfate proteoglycan of the EHS tumor, BPG-5. The deduced amino acid sequence revealed an identity of 87% between the human and the murine species. Of the 57 different amino acids encoded in the human clones, 20 were substituted with a similar amino acid. Among the specific features that were highly conserved was the 32 cysteine residues with a distinctive repeat pattern characteristic of epidermal growth factor. Northern blotting revealed a single, ∼12 kb transcript in a variety of cells including human colon carcinoma, endothelial, and fibroblastic cells. The size of this transcript correlated with the estimated molecular weight (∼400 kDa) of the protein core precursor. Southern blot analyses of DNA from 120 human/rodent somatic cell hybrids, including subclones with specific translocations or spontaneous breaks of human chromosome 1, demonstrated the presence of HSPG2 gene on the telomeric region of the short arm of chromosome 1 (1p34-pter). Two polymorphisms with TaqI and EcoRI restriction endonucleases, respectively, were also detected. The localization of this proteoglycan locus in the human genome and the availability of new RFLPs provide the tools for future studies of human diseases where the HSPG2 proteoglycan gene is suspected to be involved.

AB - We have determined the sequence of two overlapping cDNA clones encoding a portion of the human heparan sulfate proteoglycan core protein (HSPG2) from a human colon library. The cDNA clones encompassed 1.34 kb of nucleotide sequence and showed ∼85% homology to the murine heparan sulfate proteoglycan of the EHS tumor, BPG-5. The deduced amino acid sequence revealed an identity of 87% between the human and the murine species. Of the 57 different amino acids encoded in the human clones, 20 were substituted with a similar amino acid. Among the specific features that were highly conserved was the 32 cysteine residues with a distinctive repeat pattern characteristic of epidermal growth factor. Northern blotting revealed a single, ∼12 kb transcript in a variety of cells including human colon carcinoma, endothelial, and fibroblastic cells. The size of this transcript correlated with the estimated molecular weight (∼400 kDa) of the protein core precursor. Southern blot analyses of DNA from 120 human/rodent somatic cell hybrids, including subclones with specific translocations or spontaneous breaks of human chromosome 1, demonstrated the presence of HSPG2 gene on the telomeric region of the short arm of chromosome 1 (1p34-pter). Two polymorphisms with TaqI and EcoRI restriction endonucleases, respectively, were also detected. The localization of this proteoglycan locus in the human genome and the availability of new RFLPs provide the tools for future studies of human diseases where the HSPG2 proteoglycan gene is suspected to be involved.

UR - http://www.scopus.com/inward/record.url?scp=0025790413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025790413&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(91)90451-J

DO - 10.1016/0888-7543(91)90451-J

M3 - Article

C2 - 1679749

AN - SCOPUS:0025790413

VL - 10

SP - 673

EP - 680

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -