Heparan sulfate 6-O-sulfotransferase is essential for muscle development in zebrafish

Robert J. Bink, Hiroko Habuchi, Z. Lele, Edward Dolk, Jos Joore, Gerd Jörg Rauch, Robert Geisler, Stephen W. Wilson, Jeroen Den Hertog, Koji Kimata, Danica Zivkovic

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Heparan sulfate proteoglycans function in development and disease. They consist of a core protein with attached heparan sulfate chains that are altered by a series of carbohydrate-modifying enzymes and sulfotransferases. Here, we report on the identification and characterization of a gene encoding zebrafish heparan sulfate 6-O-sulfotransferase (hs6st) that shows high homology to other heparan sulfate 6-O-sulfotransferases. When expressed as a fusion protein in cultured cells, the protein shows specific 6-O-sulfotransferase activity and preferentially acts on the iduronosyl N-sulfoglycosamine. In the developing embryo, hs6st is expressed in the brain, the somites, and the fins; the same structures that were affected upon morpholino-mediated functional knockdown. Morpholino injections significantly inhibited 6-O- but not 2-O-sulfation as assessed by HPLC. Morphants display disturbed somite specification independent of the somite oscillator mechanism and have impaired muscle differentiation. In conclusion, our results show that transfer of sulfate to specific positions on glycosaminoglycans is essential for muscle development.

Original languageEnglish
Pages (from-to)31118-31127
Number of pages10
JournalJournal of Biological Chemistry
Volume278
Issue number33
DOIs
Publication statusPublished - Aug 15 2003

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Somites
Muscle Development
Zebrafish
Muscle
Sulfotransferases
Morpholinos
Heparan Sulfate Proteoglycans
Proteins
Gene encoding
Heparitin Sulfate
Glycosaminoglycans
Sulfates
Cultured Cells
Brain
Fusion reactions
Embryonic Structures
High Pressure Liquid Chromatography
Cells
Carbohydrates
Specifications

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bink, R. J., Habuchi, H., Lele, Z., Dolk, E., Joore, J., Rauch, G. J., ... Zivkovic, D. (2003). Heparan sulfate 6-O-sulfotransferase is essential for muscle development in zebrafish. Journal of Biological Chemistry, 278(33), 31118-31127. https://doi.org/10.1074/jbc.M213124200

Heparan sulfate 6-O-sulfotransferase is essential for muscle development in zebrafish. / Bink, Robert J.; Habuchi, Hiroko; Lele, Z.; Dolk, Edward; Joore, Jos; Rauch, Gerd Jörg; Geisler, Robert; Wilson, Stephen W.; Den Hertog, Jeroen; Kimata, Koji; Zivkovic, Danica.

In: Journal of Biological Chemistry, Vol. 278, No. 33, 15.08.2003, p. 31118-31127.

Research output: Contribution to journalArticle

Bink, RJ, Habuchi, H, Lele, Z, Dolk, E, Joore, J, Rauch, GJ, Geisler, R, Wilson, SW, Den Hertog, J, Kimata, K & Zivkovic, D 2003, 'Heparan sulfate 6-O-sulfotransferase is essential for muscle development in zebrafish', Journal of Biological Chemistry, vol. 278, no. 33, pp. 31118-31127. https://doi.org/10.1074/jbc.M213124200
Bink, Robert J. ; Habuchi, Hiroko ; Lele, Z. ; Dolk, Edward ; Joore, Jos ; Rauch, Gerd Jörg ; Geisler, Robert ; Wilson, Stephen W. ; Den Hertog, Jeroen ; Kimata, Koji ; Zivkovic, Danica. / Heparan sulfate 6-O-sulfotransferase is essential for muscle development in zebrafish. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 33. pp. 31118-31127.
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