Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes

Erika Gábor, Gyöngyi Cinege, Gábor Csordás, Tibor Török, Katalin Folkl-Medzihradszky, Z. Darula, I. Andó, E. Kurucz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The identification of molecular markers considerably facilitated the classification and functional analysis of blood cell types. Apis mellifera hemocytes have been classified by morphological criteria and lectin binding properties; however, the use of molecular markers has been minimal. Here we describe a monoclonal antibody to a non-phagocytic subpopulation of A. mellifera hemocytes and to a constituent of the hemolymph clot. We demonstrate that the antibody identifies the A. mellifera hemolectin, a protein carrying human von Willebrand factor homology domains, characteristic of proteins involved in blood coagulation and platelet aggregation in mammals. Hemolectin expressing A. mellifera hemocytes contain the protein as cytoplasmic granules and contribute to the formation of a protein matrix, building up around foreign particles. Consequently, hemolectin as a marker molecule reveals a clear functional heterogeneity of hemocytes, allowing for the analytical separation of hemocyte classes, and could promote the molecular identification of hemocyte lineages in A. mellifera.

Original languageEnglish
Pages (from-to)403-411
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume76
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Hemocytes
Honey
Bees
Cytoplasmic Granules
Proteins
Hemolymph
von Willebrand Factor
Blood Coagulation
Platelet Aggregation
Lectins
Mammals
Blood Cells
Blood Platelets
Monoclonal Antibodies
Antibodies

Keywords

  • Apis mellifera
  • Cellular immunity
  • Encapsulation
  • Hemocyte
  • Monoclonal antibody
  • Phagocytosis

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes. / Gábor, Erika; Cinege, Gyöngyi; Csordás, Gábor; Török, Tibor; Folkl-Medzihradszky, Katalin; Darula, Z.; Andó, I.; Kurucz, E.

In: Developmental and Comparative Immunology, Vol. 76, 01.11.2017, p. 403-411.

Research output: Contribution to journalArticle

Gábor, Erika ; Cinege, Gyöngyi ; Csordás, Gábor ; Török, Tibor ; Folkl-Medzihradszky, Katalin ; Darula, Z. ; Andó, I. ; Kurucz, E. / Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes. In: Developmental and Comparative Immunology. 2017 ; Vol. 76. pp. 403-411.
@article{7dbe71ca80c24715b787acc2b1846f15,
title = "Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes",
abstract = "The identification of molecular markers considerably facilitated the classification and functional analysis of blood cell types. Apis mellifera hemocytes have been classified by morphological criteria and lectin binding properties; however, the use of molecular markers has been minimal. Here we describe a monoclonal antibody to a non-phagocytic subpopulation of A. mellifera hemocytes and to a constituent of the hemolymph clot. We demonstrate that the antibody identifies the A. mellifera hemolectin, a protein carrying human von Willebrand factor homology domains, characteristic of proteins involved in blood coagulation and platelet aggregation in mammals. Hemolectin expressing A. mellifera hemocytes contain the protein as cytoplasmic granules and contribute to the formation of a protein matrix, building up around foreign particles. Consequently, hemolectin as a marker molecule reveals a clear functional heterogeneity of hemocytes, allowing for the analytical separation of hemocyte classes, and could promote the molecular identification of hemocyte lineages in A. mellifera.",
keywords = "Apis mellifera, Cellular immunity, Encapsulation, Hemocyte, Monoclonal antibody, Phagocytosis",
author = "Erika G{\'a}bor and Gy{\"o}ngyi Cinege and G{\'a}bor Csord{\'a}s and Tibor T{\"o}r{\"o}k and Katalin Folkl-Medzihradszky and Z. Darula and I. And{\'o} and E. Kurucz",
year = "2017",
month = "11",
day = "1",
doi = "10.1016/j.dci.2017.07.013",
language = "English",
volume = "76",
pages = "403--411",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Hemolectin expression reveals functional heterogeneity in honey bee (Apis mellifera) hemocytes

AU - Gábor, Erika

AU - Cinege, Gyöngyi

AU - Csordás, Gábor

AU - Török, Tibor

AU - Folkl-Medzihradszky, Katalin

AU - Darula, Z.

AU - Andó, I.

AU - Kurucz, E.

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The identification of molecular markers considerably facilitated the classification and functional analysis of blood cell types. Apis mellifera hemocytes have been classified by morphological criteria and lectin binding properties; however, the use of molecular markers has been minimal. Here we describe a monoclonal antibody to a non-phagocytic subpopulation of A. mellifera hemocytes and to a constituent of the hemolymph clot. We demonstrate that the antibody identifies the A. mellifera hemolectin, a protein carrying human von Willebrand factor homology domains, characteristic of proteins involved in blood coagulation and platelet aggregation in mammals. Hemolectin expressing A. mellifera hemocytes contain the protein as cytoplasmic granules and contribute to the formation of a protein matrix, building up around foreign particles. Consequently, hemolectin as a marker molecule reveals a clear functional heterogeneity of hemocytes, allowing for the analytical separation of hemocyte classes, and could promote the molecular identification of hemocyte lineages in A. mellifera.

AB - The identification of molecular markers considerably facilitated the classification and functional analysis of blood cell types. Apis mellifera hemocytes have been classified by morphological criteria and lectin binding properties; however, the use of molecular markers has been minimal. Here we describe a monoclonal antibody to a non-phagocytic subpopulation of A. mellifera hemocytes and to a constituent of the hemolymph clot. We demonstrate that the antibody identifies the A. mellifera hemolectin, a protein carrying human von Willebrand factor homology domains, characteristic of proteins involved in blood coagulation and platelet aggregation in mammals. Hemolectin expressing A. mellifera hemocytes contain the protein as cytoplasmic granules and contribute to the formation of a protein matrix, building up around foreign particles. Consequently, hemolectin as a marker molecule reveals a clear functional heterogeneity of hemocytes, allowing for the analytical separation of hemocyte classes, and could promote the molecular identification of hemocyte lineages in A. mellifera.

KW - Apis mellifera

KW - Cellular immunity

KW - Encapsulation

KW - Hemocyte

KW - Monoclonal antibody

KW - Phagocytosis

UR - http://www.scopus.com/inward/record.url?scp=85024490463&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85024490463&partnerID=8YFLogxK

U2 - 10.1016/j.dci.2017.07.013

DO - 10.1016/j.dci.2017.07.013

M3 - Article

C2 - 28713010

AN - SCOPUS:85024490463

VL - 76

SP - 403

EP - 411

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

ER -