Hemokinin-1 is an important mediator of pain in mouse models of neuropathic and inflammatory mechanisms

Ágnes Hunyady, Zsófia Hajna, Tímea Gubányi, Bálint Scheich, Ágnes Kemény, B. Gaszner, Éva Borbély, Z. Helyes

Research output: Contribution to journalArticle

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Abstract

The Tac4 gene-derived hemokinin-1 (HK-1) is present in pain-related regions and activates the tachykinin NK1 receptor, but with binding site and signaling pathways different from Substance P (SP). NK1 receptor is involved in nociception, but our earlier data showed that it has no role in chronic neuropathic hyperalgesia, similarly to SP. Furthermore, NK1 antagonists failed in clinical trials as analgesics due to still unknown reasons. Therefore, we investigated the role of HK-1 in pain conditions of distinct mechanisms using genetically modified mice. Chronic neuropathic mechanical and cold hyperalgesia after partial sciatic nerve ligation (PSL) were determined by dynamic plantar aesthesiometry and withdrawal latency from icy water, motor coordination on the accelerating Rotarod. Peripheral nerve growth factor (NGF) production was measured by ELISA, neuronal and glia cell activation by immunohistochemistry in pain-related regions. Acute somatic and visceral chemonocifensive behaviors were assessed after intraplantar formalin or intraperitoneal acetic-acid injection, respectively. Resiniferatoxin-induced inflammatory mechanical and thermal hyperalgesia by aesthesiometry and increasing temperature hot plate. Chronic neuropathic mechanical and cold hypersensitivity were significantly decreased in HK-1 deficient mice. NGF level in the paw homogenates of intact mice were significantly lower in case of HK-1 deletion. However, it significantly increased under neuropathic condition in contrast to wildtype mice, where the higher basal concentration did not show any changes. Microglia, but not astrocyte activation was observed 14 days after PSL in the ipsilateral spinal dorsal horn of wildtype, but not HK-1-deficient mice. However, under neuropathic conditions, the number of GFAP-positive astrocytes was significantly smaller in case of HK-1 deletion. Acute visceral, but not somatic nocifensive behavior, as well as neurogenic inflammatory mechanical and thermal hypersensitivity were significantly reduced by HK-1 deficiency similarly to NK1, but not to SP deletion. We provide evidence for pro-nociceptive role of HK-1, via NK1 receptor activation in acute inflammation models, but differently from SP-mediated actions. Identification of its targets and signaling can open new directions in pain research.

Original languageEnglish
Pages (from-to)165-173
Number of pages9
JournalBrain Research Bulletin
Volume147
DOIs
Publication statusPublished - Apr 1 2019

Fingerprint

Hyperalgesia
Substance P
Pain
Nerve Growth Factor
Sciatic Nerve
Astrocytes
Ligation
Hot Temperature
Tachykinin Receptors
Nociception
Microglia
Neuroglia
Acetic Acid
Formaldehyde
Analgesics
hemokinin-1
Hypersensitivity
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Binding Sites

Keywords

  • Formalin test
  • Microglia
  • NGF
  • NK1 receptor
  • Partial sciatic nerve ligation
  • Resiniferatoxin-induced hypersensitivity
  • Tachykinins
  • Writhing test

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hemokinin-1 is an important mediator of pain in mouse models of neuropathic and inflammatory mechanisms. / Hunyady, Ágnes; Hajna, Zsófia; Gubányi, Tímea; Scheich, Bálint; Kemény, Ágnes; Gaszner, B.; Borbély, Éva; Helyes, Z.

In: Brain Research Bulletin, Vol. 147, 01.04.2019, p. 165-173.

Research output: Contribution to journalArticle

Hunyady, Ágnes ; Hajna, Zsófia ; Gubányi, Tímea ; Scheich, Bálint ; Kemény, Ágnes ; Gaszner, B. ; Borbély, Éva ; Helyes, Z. / Hemokinin-1 is an important mediator of pain in mouse models of neuropathic and inflammatory mechanisms. In: Brain Research Bulletin. 2019 ; Vol. 147. pp. 165-173.
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