Hemese, a hemocyte-specific transmembrane protein, affects the cellular immune response in Drosophila

Eva Kurucz, Carl Johan Zettervall, Rita Sinka, Peter Vilmos, Andor Pivarcsi, Sophia Ekengren, Zoltán Hegedüs, Istvan Ando, Dan Hultmark

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

We have identified a previously undescribed transmembrane protein, Hemese, from Drosophila melanogaster blood cells (hemocytes), by using a monoclonal pan-hemocyte antibody. Heavy glycosylation is suggested by the heterogeneous size distribution, ranging between 37 and 70 kDa. Hemese expression is restricted to the cell surfaces of hemocytes of all classes, and to the hematopoietic organs. The sequence of the corresponding gene, Hemese (He), predicts a glycophorin-like protein of 15 kDa, excluding an N-terminal signal peptide, with a single hydrophobic transmembrane region. The extracellular region consists mainly of Ser/Thr-rich sequence of low complexity, with several potential O-glycosylation sites. Hemese contains phosphotyrosine and the cytoplasmic region has potential phosphorylation sites, suggesting an involvement in signal transduction. Depletion of Hemese by RNA interference has no obvious effect under normal conditions, but the cellular response to parasitic wasps is much enhanced. This finding indicates that Hemese plays a modulatory role in the activation or recruitment of the hemocytes.

Original languageEnglish
Pages (from-to)2622-2627
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number5
DOIs
Publication statusPublished - Mar 4 2003

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Hemocytes
Cellular Immunity
Drosophila
Glycosylation
Proteins
Glycophorin
Drosophila Proteins
Wasps
Phosphotyrosine
Protein Sorting Signals
RNA Interference
Signal Transduction
Blood Cells
Phosphorylation
Antibodies
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Hemese, a hemocyte-specific transmembrane protein, affects the cellular immune response in Drosophila. / Kurucz, Eva; Zettervall, Carl Johan; Sinka, Rita; Vilmos, Peter; Pivarcsi, Andor; Ekengren, Sophia; Hegedüs, Zoltán; Ando, Istvan; Hultmark, Dan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 5, 04.03.2003, p. 2622-2627.

Research output: Contribution to journalArticle

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AU - Kurucz, Eva

AU - Zettervall, Carl Johan

AU - Sinka, Rita

AU - Vilmos, Peter

AU - Pivarcsi, Andor

AU - Ekengren, Sophia

AU - Hegedüs, Zoltán

AU - Ando, Istvan

AU - Hultmark, Dan

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