Heme oxygenase-1-related carbon monoxide and flavonoids in ischemic/reperfused rat retina

Marta E. Szabo, Eva Gallyas, I. Bak, Andry Rakotovao, Francois Boucher, Joel De Leiris, Norbert Nagy, E. Varga, A. Tósaki

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

PURPOSE. There is increasing evidence to show cytoprotective effects of various flavonoid-rich extracts and the tissue-protective capacity of flavonoid-rich extract of sour cherry is due to flavonoid components of seeds. Sour cherry seed flavonoids were evaluated for their contribution to postischemic recovery related to endogenous carbon monoxide (CO) production in rat retinas subjected to ischemia/reperfusion. METHODS. Rats were orally treated with selected doses of flavonoid-rich extract of sour cherry seeds for 2 weeks. Animals were anesthetized, and a suture was placed behind the globe including the central retinal artery. Next, retinas were subjected to 90 minutes of ischemia followed by 24 hours of reperfusion. After this procedure, heme oxygenase-1 (HO-1)-related protein expression and enzyme activity, HO-1-related endogenous CO production, and ionic imbalance including tissue Na+, K+, and Ca2+ in untreated and treated ischemic/reperfused retinas were measured. RESULTS. Retinal ischemia/reperfusion resulted in a significant reduction (to 10%) in HO-1 protein expression, enzyme activity, and HO-1-related endogenous CO production in the retina. These changes were accompanied by increases in retinal Na+ and Ca2+ gains and loss of K+. In rats treated with 10 and 30 mg/kg of sour cherry flavonoid-rich extract, after 24 hours of reperfusion, tissue Na+ and Ca2+accumulation and K+ loss were prevented in comparison with the drug-free control. CONCLUSIONS. Sour cherry seed flavonoid-rich extract showed a protective effect against reperfusion-induced injury through its ability to reduce the changes in concentrations of retinal ions through HO-1-related endogenous CO production in the ischemic/reperfused retina.

Original languageEnglish
Pages (from-to)3727-3732
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume45
Issue number10
DOIs
Publication statusPublished - Oct 2004

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Heme Oxygenase-1
Carbon Monoxide
Flavonoids
Retina
Reperfusion
Seeds
Ischemia
Retinal Artery
Tissue Extracts
Drug and Narcotic Control
Enzymes
Reperfusion Injury
Sutures
Proteins
Prunus avium
Ions

ASJC Scopus subject areas

  • Ophthalmology

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Heme oxygenase-1-related carbon monoxide and flavonoids in ischemic/reperfused rat retina. / Szabo, Marta E.; Gallyas, Eva; Bak, I.; Rakotovao, Andry; Boucher, Francois; De Leiris, Joel; Nagy, Norbert; Varga, E.; Tósaki, A.

In: Investigative Ophthalmology and Visual Science, Vol. 45, No. 10, 10.2004, p. 3727-3732.

Research output: Contribution to journalArticle

Szabo, Marta E. ; Gallyas, Eva ; Bak, I. ; Rakotovao, Andry ; Boucher, Francois ; De Leiris, Joel ; Nagy, Norbert ; Varga, E. ; Tósaki, A. / Heme oxygenase-1-related carbon monoxide and flavonoids in ischemic/reperfused rat retina. In: Investigative Ophthalmology and Visual Science. 2004 ; Vol. 45, No. 10. pp. 3727-3732.
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abstract = "PURPOSE. There is increasing evidence to show cytoprotective effects of various flavonoid-rich extracts and the tissue-protective capacity of flavonoid-rich extract of sour cherry is due to flavonoid components of seeds. Sour cherry seed flavonoids were evaluated for their contribution to postischemic recovery related to endogenous carbon monoxide (CO) production in rat retinas subjected to ischemia/reperfusion. METHODS. Rats were orally treated with selected doses of flavonoid-rich extract of sour cherry seeds for 2 weeks. Animals were anesthetized, and a suture was placed behind the globe including the central retinal artery. Next, retinas were subjected to 90 minutes of ischemia followed by 24 hours of reperfusion. After this procedure, heme oxygenase-1 (HO-1)-related protein expression and enzyme activity, HO-1-related endogenous CO production, and ionic imbalance including tissue Na+, K+, and Ca2+ in untreated and treated ischemic/reperfused retinas were measured. RESULTS. Retinal ischemia/reperfusion resulted in a significant reduction (to 10{\%}) in HO-1 protein expression, enzyme activity, and HO-1-related endogenous CO production in the retina. These changes were accompanied by increases in retinal Na+ and Ca2+ gains and loss of K+. In rats treated with 10 and 30 mg/kg of sour cherry flavonoid-rich extract, after 24 hours of reperfusion, tissue Na+ and Ca2+accumulation and K+ loss were prevented in comparison with the drug-free control. CONCLUSIONS. Sour cherry seed flavonoid-rich extract showed a protective effect against reperfusion-induced injury through its ability to reduce the changes in concentrations of retinal ions through HO-1-related endogenous CO production in the ischemic/reperfused retina.",
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AU - Szabo, Marta E.

AU - Gallyas, Eva

AU - Bak, I.

AU - Rakotovao, Andry

AU - Boucher, Francois

AU - De Leiris, Joel

AU - Nagy, Norbert

AU - Varga, E.

AU - Tósaki, A.

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N2 - PURPOSE. There is increasing evidence to show cytoprotective effects of various flavonoid-rich extracts and the tissue-protective capacity of flavonoid-rich extract of sour cherry is due to flavonoid components of seeds. Sour cherry seed flavonoids were evaluated for their contribution to postischemic recovery related to endogenous carbon monoxide (CO) production in rat retinas subjected to ischemia/reperfusion. METHODS. Rats were orally treated with selected doses of flavonoid-rich extract of sour cherry seeds for 2 weeks. Animals were anesthetized, and a suture was placed behind the globe including the central retinal artery. Next, retinas were subjected to 90 minutes of ischemia followed by 24 hours of reperfusion. After this procedure, heme oxygenase-1 (HO-1)-related protein expression and enzyme activity, HO-1-related endogenous CO production, and ionic imbalance including tissue Na+, K+, and Ca2+ in untreated and treated ischemic/reperfused retinas were measured. RESULTS. Retinal ischemia/reperfusion resulted in a significant reduction (to 10%) in HO-1 protein expression, enzyme activity, and HO-1-related endogenous CO production in the retina. These changes were accompanied by increases in retinal Na+ and Ca2+ gains and loss of K+. In rats treated with 10 and 30 mg/kg of sour cherry flavonoid-rich extract, after 24 hours of reperfusion, tissue Na+ and Ca2+accumulation and K+ loss were prevented in comparison with the drug-free control. CONCLUSIONS. Sour cherry seed flavonoid-rich extract showed a protective effect against reperfusion-induced injury through its ability to reduce the changes in concentrations of retinal ions through HO-1-related endogenous CO production in the ischemic/reperfused retina.

AB - PURPOSE. There is increasing evidence to show cytoprotective effects of various flavonoid-rich extracts and the tissue-protective capacity of flavonoid-rich extract of sour cherry is due to flavonoid components of seeds. Sour cherry seed flavonoids were evaluated for their contribution to postischemic recovery related to endogenous carbon monoxide (CO) production in rat retinas subjected to ischemia/reperfusion. METHODS. Rats were orally treated with selected doses of flavonoid-rich extract of sour cherry seeds for 2 weeks. Animals were anesthetized, and a suture was placed behind the globe including the central retinal artery. Next, retinas were subjected to 90 minutes of ischemia followed by 24 hours of reperfusion. After this procedure, heme oxygenase-1 (HO-1)-related protein expression and enzyme activity, HO-1-related endogenous CO production, and ionic imbalance including tissue Na+, K+, and Ca2+ in untreated and treated ischemic/reperfused retinas were measured. RESULTS. Retinal ischemia/reperfusion resulted in a significant reduction (to 10%) in HO-1 protein expression, enzyme activity, and HO-1-related endogenous CO production in the retina. These changes were accompanied by increases in retinal Na+ and Ca2+ gains and loss of K+. In rats treated with 10 and 30 mg/kg of sour cherry flavonoid-rich extract, after 24 hours of reperfusion, tissue Na+ and Ca2+accumulation and K+ loss were prevented in comparison with the drug-free control. CONCLUSIONS. Sour cherry seed flavonoid-rich extract showed a protective effect against reperfusion-induced injury through its ability to reduce the changes in concentrations of retinal ions through HO-1-related endogenous CO production in the ischemic/reperfused retina.

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