Heme oxygenase 1 (HMOX1) gene expression in hemodialysed uremic patients

Zoltán Maróti, Sándor Túri, Ilona Németh, Eszter Karg, Péter Ugocsai, Emoke Endreffy

Research output: Contribution to journalArticle

Abstract

The oxidative stress and the released heme molecule of degrading red blood cells during hemodialysis induce the expression of heme oxygenase 1 gene (HMOX1). The goal of our study was to measure the expression of HMOX1 during dialysis and compare it with the common oxidative markers. We established a cRT-PCR (competitive reverse transcriptase PCR) method to measure the mRNA levels. The mRNA levels were calculated to the white blood cell count respectively for each sample. We measured a 1-5-fold increase in the HMOX1 gene expression after the dialysis session, which dropped back to the original value 48 hours after the dialysis. The base level of HMOX1 mRNA of hemodialysed patients before dialysis was in the range of normal controls. The changes in mRNA levels showed significant correlation with the plasma hemoglobin (r=0.72, p<0.001) and plasma bilirubin (r=0.71, p<0.002) changes. We found no correlation between the changes of mRNA levels and other biochemical markers (GSH, GSSG). Our results suggest that the induced HMOX1 expression during hemodialysis is primarily due to the undergoing hemolysis.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalActa Biologica Szegediensis
Volume47
Issue number1-4
Publication statusPublished - Dec 1 2003

Keywords

  • Gene expression
  • Heme oxygenase 1
  • Hemodialysis
  • Oxidative stress
  • cRT-PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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