Hematological and hemostaseological alterations after warm and cold limb ischemia-reperfusion in a canine model

Research output: Contribution to journalArticle

6 Citations (Scopus)


Purpose: Acute ischemia-reperfusion (I/R) of extremities means serious challenge in the clinical practice. Furthermore, the issue of preventive cooling is still controversial. In this canine model we investigated whether limb I/R -with or without cooling- has an influence on hematological and hemostaseological factors. Methods: Femoral vessels were exposed and clamped for 3 hours. After release the clamps, 4-hour reperfusion was secured. The same procedures with cooling using ice bags, as well as warm and cold sham-operations were performed. Before operations, from the excluded limb by the end of ischemia, during the reperfusion, and for 5 postoperative days afterwards blood samples were collected for testing hematological and blood coagulation parameters. Results: After I/R activated partial thromboplastin time was elongated on 2nd-4th postoperative days. The highest values were on the 2nd day in cold I/R group, accompanied by increased prothrombin time values. The hematological parameters and fibrinogen level showed non-specific changes. In excluded ischemic limb the blood composition showed controversial data. Cold ischemia induced larger alterations, however platelet count, hematocrit changed more expressly in warm ischemia. Conclusion: These results indicate the risk of coagulopathy following limb I/R on early post-eventually days, which risk is higher in the case of cold I/R.

Original languageEnglish
Pages (from-to)338-346
Number of pages9
JournalActa Cirurgica Brasileira
Issue number5
Publication statusPublished - Sep 1 2009



  • Blood coagulation
  • Cooling
  • Dogs
  • Hematology
  • Ischemia
  • Lower extremity
  • Reperfusion

ASJC Scopus subject areas

  • Surgery

Cite this