Helicobacter pylori lipopolysaccharide-provoked injury to rat gastroduodenal microvasculature involves inducible nitric oxide synthase

József Kiss, Dominique Lamarque, Anthony P. Moran, József Pozsár, E. Morschl, F. László, Brendan J R Whittle

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The actions of a purified Helicobacter pylori lipopolysaccharide (3 mg kg-1, i.v.) on rat gastric antral and duodenal microvascular integrity (determined as radiolabelled albumin leakage) and the expression of the inducible nitric oxide (NO) synthase (iNOS; assessed by the citrulline assay) were investigated 4 h after challenge. Significant increases of albumin leakage and expression of iNOS in both antral and duodenal tissues were observed following challenge. Concurrent administration of the selective iNOS inhibitor, 1400W (N-(8-(aminomethyl)benzyl)-acetamidine; 0.2-1 mg kg-1, s.c.), with lipopolysaccharide, caused a dose-dependent attenuation of the gastric and duodenal albumin leakage. Thus, H. pylori lipopolysaccharide can initiate the expression of iNOS in the stomach and duodenum following systemic challenge, which can provoke gastroduodenal microvascular dysfunction.

Original languageEnglish
Pages (from-to)175-179
Number of pages5
JournalEuropean Journal of Pharmacology
Volume420
Issue number2-3
DOIs
Publication statusPublished - May 25 2001

Fingerprint

Nitric Oxide Synthase Type II
Microvessels
Albumins
Stomach
Wounds and Injuries
Citrulline
Duodenum
Lipopolysaccharides
Helicobacter pylori lipopolysaccharide
Antral

Keywords

  • Duodenum
  • Gastritis
  • Helicobacter pylori
  • Inducible
  • Inflammation
  • Lipopolysaccharide
  • Nitric oxide (NO) synthase
  • Nitric oxide (NO) synthase inhibitor

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Helicobacter pylori lipopolysaccharide-provoked injury to rat gastroduodenal microvasculature involves inducible nitric oxide synthase. / Kiss, József; Lamarque, Dominique; Moran, Anthony P.; Pozsár, József; Morschl, E.; László, F.; Whittle, Brendan J R.

In: European Journal of Pharmacology, Vol. 420, No. 2-3, 25.05.2001, p. 175-179.

Research output: Contribution to journalArticle

Kiss, József ; Lamarque, Dominique ; Moran, Anthony P. ; Pozsár, József ; Morschl, E. ; László, F. ; Whittle, Brendan J R. / Helicobacter pylori lipopolysaccharide-provoked injury to rat gastroduodenal microvasculature involves inducible nitric oxide synthase. In: European Journal of Pharmacology. 2001 ; Vol. 420, No. 2-3. pp. 175-179.
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