Helicobacter pylori infection in connective tissue disorders is associated with high levels of antibodies to mycobacterial hsp65 but not to human hsp60

L. Kalabay, B. Fekete, L. Czirják, L. Horváth, Mohamed R. Daha, A. Veres, Gábor Fónyad, A. Horváth, Ágnes Viczián, Mahavir Singh, Izabella Hoffer, G. Füst, László Romics, Z. Prohászka

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background. To investigate whether the Helicobacter pylori status influences levels of antibodies against mycobacterial heat shock protein (hsp) 65 and human hsp60 in systemic autoimmune diseases and to study the concentration of anti-H. pylori antibodies in autoimmune patients and healthy controls. Materials and Methods. Antibodies against human heat-shock protein hsp60, mycobacterial heat-shock protein hsp65 were analyzed by ELISA. Anti-Helicobacter antibodies were determined by enzyme immunoassay. Results. There was a markedly higher prevalence of H. pylori infection in undifferentiated connective tissue disease (82%) (n = 33) and systemic sclerosis (78%) (n = 55) but not in systemic lupus erythematosus (n = 49), polymyositis/dermatomyositis (n = 14), rheumatoid arthritis (n = 21) or primary Raynaud's syndrome (n = 26) compared with controls (59%) (n = 349). In autoimmune diseases H. pylori infection was associated with elevated levels of antihsp65 (p = .008) but not of antihsp60. Anti-hsp65 levels were significantly higher in H. pylori-infected (n = 129) than in uninfected patients (n = 69) (p = .0007). Conclusions. These findings indicate that in autoimmune diseases the infection with the H. pylori bacterium is associated with increased concentration of antimycobacterial hsp65.

Original languageEnglish
Pages (from-to)250-256
Number of pages7
JournalHelicobacter
Volume7
Issue number4
DOIs
Publication statusPublished - 2002

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Helicobacter Infections
Helicobacter pylori
Connective Tissue
Antibodies
Autoimmune Diseases
Heat-Shock Proteins
Helicobacter
Connective Tissue Diseases
Dermatomyositis
Systemic Scleroderma
Immunoenzyme Techniques
Systemic Lupus Erythematosus
Anti-Idiotypic Antibodies
Rheumatoid Arthritis
Enzyme-Linked Immunosorbent Assay
Bacteria
Infection

Keywords

  • Anti-human hsp60 antibody
  • Antimycobacterial hsp65 antibody
  • Helicobacter pylori
  • Systemic autoimmune disease

ASJC Scopus subject areas

  • Gastroenterology
  • Microbiology

Cite this

Helicobacter pylori infection in connective tissue disorders is associated with high levels of antibodies to mycobacterial hsp65 but not to human hsp60. / Kalabay, L.; Fekete, B.; Czirják, L.; Horváth, L.; Daha, Mohamed R.; Veres, A.; Fónyad, Gábor; Horváth, A.; Viczián, Ágnes; Singh, Mahavir; Hoffer, Izabella; Füst, G.; Romics, László; Prohászka, Z.

In: Helicobacter, Vol. 7, No. 4, 2002, p. 250-256.

Research output: Contribution to journalArticle

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title = "Helicobacter pylori infection in connective tissue disorders is associated with high levels of antibodies to mycobacterial hsp65 but not to human hsp60",
abstract = "Background. To investigate whether the Helicobacter pylori status influences levels of antibodies against mycobacterial heat shock protein (hsp) 65 and human hsp60 in systemic autoimmune diseases and to study the concentration of anti-H. pylori antibodies in autoimmune patients and healthy controls. Materials and Methods. Antibodies against human heat-shock protein hsp60, mycobacterial heat-shock protein hsp65 were analyzed by ELISA. Anti-Helicobacter antibodies were determined by enzyme immunoassay. Results. There was a markedly higher prevalence of H. pylori infection in undifferentiated connective tissue disease (82{\%}) (n = 33) and systemic sclerosis (78{\%}) (n = 55) but not in systemic lupus erythematosus (n = 49), polymyositis/dermatomyositis (n = 14), rheumatoid arthritis (n = 21) or primary Raynaud's syndrome (n = 26) compared with controls (59{\%}) (n = 349). In autoimmune diseases H. pylori infection was associated with elevated levels of antihsp65 (p = .008) but not of antihsp60. Anti-hsp65 levels were significantly higher in H. pylori-infected (n = 129) than in uninfected patients (n = 69) (p = .0007). Conclusions. These findings indicate that in autoimmune diseases the infection with the H. pylori bacterium is associated with increased concentration of antimycobacterial hsp65.",
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author = "L. Kalabay and B. Fekete and L. Czirj{\'a}k and L. Horv{\'a}th and Daha, {Mohamed R.} and A. Veres and G{\'a}bor F{\'o}nyad and A. Horv{\'a}th and {\'A}gnes Viczi{\'a}n and Mahavir Singh and Izabella Hoffer and G. F{\"u}st and L{\'a}szl{\'o} Romics and Z. Proh{\'a}szka",
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T1 - Helicobacter pylori infection in connective tissue disorders is associated with high levels of antibodies to mycobacterial hsp65 but not to human hsp60

AU - Kalabay, L.

AU - Fekete, B.

AU - Czirják, L.

AU - Horváth, L.

AU - Daha, Mohamed R.

AU - Veres, A.

AU - Fónyad, Gábor

AU - Horváth, A.

AU - Viczián, Ágnes

AU - Singh, Mahavir

AU - Hoffer, Izabella

AU - Füst, G.

AU - Romics, László

AU - Prohászka, Z.

PY - 2002

Y1 - 2002

N2 - Background. To investigate whether the Helicobacter pylori status influences levels of antibodies against mycobacterial heat shock protein (hsp) 65 and human hsp60 in systemic autoimmune diseases and to study the concentration of anti-H. pylori antibodies in autoimmune patients and healthy controls. Materials and Methods. Antibodies against human heat-shock protein hsp60, mycobacterial heat-shock protein hsp65 were analyzed by ELISA. Anti-Helicobacter antibodies were determined by enzyme immunoassay. Results. There was a markedly higher prevalence of H. pylori infection in undifferentiated connective tissue disease (82%) (n = 33) and systemic sclerosis (78%) (n = 55) but not in systemic lupus erythematosus (n = 49), polymyositis/dermatomyositis (n = 14), rheumatoid arthritis (n = 21) or primary Raynaud's syndrome (n = 26) compared with controls (59%) (n = 349). In autoimmune diseases H. pylori infection was associated with elevated levels of antihsp65 (p = .008) but not of antihsp60. Anti-hsp65 levels were significantly higher in H. pylori-infected (n = 129) than in uninfected patients (n = 69) (p = .0007). Conclusions. These findings indicate that in autoimmune diseases the infection with the H. pylori bacterium is associated with increased concentration of antimycobacterial hsp65.

AB - Background. To investigate whether the Helicobacter pylori status influences levels of antibodies against mycobacterial heat shock protein (hsp) 65 and human hsp60 in systemic autoimmune diseases and to study the concentration of anti-H. pylori antibodies in autoimmune patients and healthy controls. Materials and Methods. Antibodies against human heat-shock protein hsp60, mycobacterial heat-shock protein hsp65 were analyzed by ELISA. Anti-Helicobacter antibodies were determined by enzyme immunoassay. Results. There was a markedly higher prevalence of H. pylori infection in undifferentiated connective tissue disease (82%) (n = 33) and systemic sclerosis (78%) (n = 55) but not in systemic lupus erythematosus (n = 49), polymyositis/dermatomyositis (n = 14), rheumatoid arthritis (n = 21) or primary Raynaud's syndrome (n = 26) compared with controls (59%) (n = 349). In autoimmune diseases H. pylori infection was associated with elevated levels of antihsp65 (p = .008) but not of antihsp60. Anti-hsp65 levels were significantly higher in H. pylori-infected (n = 129) than in uninfected patients (n = 69) (p = .0007). Conclusions. These findings indicate that in autoimmune diseases the infection with the H. pylori bacterium is associated with increased concentration of antimycobacterial hsp65.

KW - Anti-human hsp60 antibody

KW - Antimycobacterial hsp65 antibody

KW - Helicobacter pylori

KW - Systemic autoimmune disease

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