Helically Organized Macroaggregates of Pigment-Protein Complexes in Chloroplasts: Evidence from Circular Intensity Differential Scattering

Gyozo Garab, Sam Wells, Laura Finzi, Carlos Bustamante

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43 Citations (Scopus)

Abstract

Angle dependence of circular intensity differential scattering (CIDS) and of nonpolarized scattering was determined in isolated spinach chloroplasts at 514.5 nm. CIDS between 0° and 170° was independent of the nonpolarized scattering and showed intense lobes of alternating signs, exhibiting the negative and positive maxima around 15° and 70°, respectively. These results provide experimental evidence for the existence of large helically organized macroaggregates of pigment-protein complexes in thylakoid membranes. Modeling of the CIDS data by a simple helical array of uniaxial polarizable groups suggests that the chiral structure is left-handed with pitch and radius of the order of 385 nm.

Original languageEnglish
Pages (from-to)5839-5843
Number of pages5
JournalBiochemistry
Volume27
Issue number16
DOIs
Publication statusPublished - Aug 1 1988

ASJC Scopus subject areas

  • Biochemistry

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