Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field

L. Bodrossy, J. C. Murrell, H. Dalton, M. Kalman, L. G. Puskas, K. L. Kovacs

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Abstract

Methanotrophic bacteria were isolated from a natural environment potentially favorable to heat-tolerant methanotrophs. An improved colony plate assay was developed and used to identify putative methanotrophic colonies with high confidence. Fourteen new isolates were purified and partially characterized. These new isolates exhibit a DNA sequence homology of up to 97% with the conserved regions in the mmoX and mmoC genes of the soluble methane monooxygenase (MMO)-coding gene cluster of Methylococcus capsulatus Bath. The copper regulation of soluble MMO expression in the same isolates, however, differs from that of M. capsulatus Bath, as the new isolates can tolerate up to 0.8 μM copper without loss of MMO activity while a drastic reduction of MMO activity occurs already at 0.1 μM copper in M. capsulatus Bath. The isolates can be cultivated and utilized at elevated temperatures, and their copper- and heat-tolerant MMO activity makes these bacteria ideal candidates for future biotechnological use.

Original languageEnglish
Pages (from-to)3549-3555
Number of pages7
JournalApplied and environmental microbiology
Volume61
Issue number10
Publication statusPublished - Jan 1 1995

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ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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