Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field

L. Bodrossy, J. C. Murrell, H. Dalton, M. Kalman, L. Puskás, K. Kovács

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Methanotrophic bacteria were isolated from a natural environment potentially favorable to heat-tolerant methanotrophs. An improved colony plate assay was developed and used to identify putative methanotrophic colonies with high confidence. Fourteen new isolates were purified and partially characterized. These new isolates exhibit a DNA sequence homology of up to 97% with the conserved regions in the mmoX and mmoC genes of the soluble methane monooxygenase (MMO)-coding gene cluster of Methylococcus capsulatus Bath. The copper regulation of soluble MMO expression in the same isolates, however, differs from that of M. capsulatus Bath, as the new isolates can tolerate up to 0.8 μM copper without loss of MMO activity while a drastic reduction of MMO activity occurs already at 0.1 μM copper in M. capsulatus Bath. The isolates can be cultivated and utilized at elevated temperatures, and their copper- and heat-tolerant MMO activity makes these bacteria ideal candidates for future biotechnological use.

Original languageEnglish
Pages (from-to)3549-3555
Number of pages7
JournalApplied and Environmental Microbiology
Volume61
Issue number10
Publication statusPublished - 1995

Fingerprint

methane monooxygenase
Natural Gas
Oil and Gas Fields
Methylococcus capsulatus
natural gas
gas field
effluents
Hot Temperature
copper
methane
effluent
Copper
Bacteria
heat
bacterium
Baths
Water
bacteria
water
methanotrophs

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field. / Bodrossy, L.; Murrell, J. C.; Dalton, H.; Kalman, M.; Puskás, L.; Kovács, K.

In: Applied and Environmental Microbiology, Vol. 61, No. 10, 1995, p. 3549-3555.

Research output: Contribution to journalArticle

Bodrossy, L. ; Murrell, J. C. ; Dalton, H. ; Kalman, M. ; Puskás, L. ; Kovács, K. / Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field. In: Applied and Environmental Microbiology. 1995 ; Vol. 61, No. 10. pp. 3549-3555.
@article{5a69bc76768f4804928dcfb4684e843e,
title = "Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field",
abstract = "Methanotrophic bacteria were isolated from a natural environment potentially favorable to heat-tolerant methanotrophs. An improved colony plate assay was developed and used to identify putative methanotrophic colonies with high confidence. Fourteen new isolates were purified and partially characterized. These new isolates exhibit a DNA sequence homology of up to 97{\%} with the conserved regions in the mmoX and mmoC genes of the soluble methane monooxygenase (MMO)-coding gene cluster of Methylococcus capsulatus Bath. The copper regulation of soluble MMO expression in the same isolates, however, differs from that of M. capsulatus Bath, as the new isolates can tolerate up to 0.8 μM copper without loss of MMO activity while a drastic reduction of MMO activity occurs already at 0.1 μM copper in M. capsulatus Bath. The isolates can be cultivated and utilized at elevated temperatures, and their copper- and heat-tolerant MMO activity makes these bacteria ideal candidates for future biotechnological use.",
author = "L. Bodrossy and Murrell, {J. C.} and H. Dalton and M. Kalman and L. Pusk{\'a}s and K. Kov{\'a}cs",
year = "1995",
language = "English",
volume = "61",
pages = "3549--3555",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field

AU - Bodrossy, L.

AU - Murrell, J. C.

AU - Dalton, H.

AU - Kalman, M.

AU - Puskás, L.

AU - Kovács, K.

PY - 1995

Y1 - 1995

N2 - Methanotrophic bacteria were isolated from a natural environment potentially favorable to heat-tolerant methanotrophs. An improved colony plate assay was developed and used to identify putative methanotrophic colonies with high confidence. Fourteen new isolates were purified and partially characterized. These new isolates exhibit a DNA sequence homology of up to 97% with the conserved regions in the mmoX and mmoC genes of the soluble methane monooxygenase (MMO)-coding gene cluster of Methylococcus capsulatus Bath. The copper regulation of soluble MMO expression in the same isolates, however, differs from that of M. capsulatus Bath, as the new isolates can tolerate up to 0.8 μM copper without loss of MMO activity while a drastic reduction of MMO activity occurs already at 0.1 μM copper in M. capsulatus Bath. The isolates can be cultivated and utilized at elevated temperatures, and their copper- and heat-tolerant MMO activity makes these bacteria ideal candidates for future biotechnological use.

AB - Methanotrophic bacteria were isolated from a natural environment potentially favorable to heat-tolerant methanotrophs. An improved colony plate assay was developed and used to identify putative methanotrophic colonies with high confidence. Fourteen new isolates were purified and partially characterized. These new isolates exhibit a DNA sequence homology of up to 97% with the conserved regions in the mmoX and mmoC genes of the soluble methane monooxygenase (MMO)-coding gene cluster of Methylococcus capsulatus Bath. The copper regulation of soluble MMO expression in the same isolates, however, differs from that of M. capsulatus Bath, as the new isolates can tolerate up to 0.8 μM copper without loss of MMO activity while a drastic reduction of MMO activity occurs already at 0.1 μM copper in M. capsulatus Bath. The isolates can be cultivated and utilized at elevated temperatures, and their copper- and heat-tolerant MMO activity makes these bacteria ideal candidates for future biotechnological use.

UR - http://www.scopus.com/inward/record.url?scp=0028799251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028799251&partnerID=8YFLogxK

M3 - Article

VL - 61

SP - 3549

EP - 3555

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

ER -