Heat shock protein synthesis of the cyanobacterium Synechocystis PCC 6803: purification of the GroEL-related chaperonin

Csaba Lehel, Hajime Wada, Eszter Kovács, Z. Török, Z. Gombos, I. Horváth, Norio Murata, L. Vígh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Synechocystis PCC 6803 cells could be induced to synthesize four major HSPs with apparent molecular sizes of 70, 64, 15 and 14 kDa. Heat stress at 42.5 °C appeared to be the optimum temperature for HSP formation in cells grown at 30 °C. The relative rate of synthesis of HSP70 and HSP15 reached a maximum at 30 min after the temperature shift-up whereas the capability of cells to accumulate HSP64 and HSP14 continued through 2 h. The two most abundant HSPs, HSP70 and HSP64, were recognized on western blots by antibodies raised against authentic DnaK and GroEL from Escherichia coli. To furnish sufficient evidence for the assumption that HSP64 is a GroEL-related chaperonin, this protein was purified to homogeneity. There was a 76% sequence identity between the amino acid sequence of HSP64 and the corresponding protein in Synechococcus PCC 7942. Moreover, the purified HSP64 cross-reacted to anti-E. coli GroEL antibody. To our knowledge, this is the first report about the purification and partial protein sequencing of a cyanobacterial chaperonin.

Original languageEnglish
Pages (from-to)327-336
Number of pages10
JournalPlant Molecular Biology
Volume18
Issue number2
DOIs
Publication statusPublished - Jan 1992

Fingerprint

Chaperonins
chaperonins
Synechocystis
Cyanobacteria
Heat-Shock Proteins
heat shock proteins
Purification
protein synthesis
Escherichia coli
Synechococcus
Proteins
Temperature
antibodies
proteins
Antibodies
Protein Sequence Analysis
cells
heat stress
Amino Acid Sequence
temperature

Keywords

  • chaperonin
  • heat shock protein (HSP)
  • HSP64
  • HSP70
  • Rubisco-binding protein
  • Synechocystis PCC 6803

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Heat shock protein synthesis of the cyanobacterium Synechocystis PCC 6803 : purification of the GroEL-related chaperonin. / Lehel, Csaba; Wada, Hajime; Kovács, Eszter; Török, Z.; Gombos, Z.; Horváth, I.; Murata, Norio; Vígh, L.

In: Plant Molecular Biology, Vol. 18, No. 2, 01.1992, p. 327-336.

Research output: Contribution to journalArticle

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AU - Török, Z.

AU - Gombos, Z.

AU - Horváth, I.

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