"Heat shock lipid" in cyanobacteria during heat/light-acclimation

Zsolt Balogi, Z. Török, G. Balogh, Katalin Jósvay, Natalia Shigapova, Elizabeth Vierling, L. Vígh, I. Horváth

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Parallel with the heat/light-induced thylakoid microdomain reorganization and thermal stabilization of photosynthesis we observed an increase in the level of the highly saturated monoglucosyldiacylglycerol (MGlcDG) in Synechocystis cells. The unusually high microviscosity obtained in thylakoid MGlcDG liposomes by monitoring DPH anisotropy was in good agreement with its exceptionally high acyl chain saturation. The MGlcDG membranes remained stable even at extreme high temperatures. Strikingly, in monolayer experiments, out of the five thylakoid polar lipids tested, MGlcDG expressed the strongest interaction with the thylakoid-stabilizing small Hsp from Synechocystis, Hsp17. The preferential interaction of Hsp17 with non-bilayer phase forming lipids supports our notion that sHsps counteract the formation of thermally induced local non-bilayer structures [Proc. Natl. Acad. Sci. USA 99 (2002) 13504] and thus implicated in microdomain organization and in the preservation of functional integrity of thylakoid membranes challenged by heat stress in the light. We also suggest that the highly saturated MGlcDG functions as a "heat shock lipid" and is of potential importance in the development of acquired thermotolerance of heat/light-primed cyanobacterial thylakoids.

Original languageEnglish
Pages (from-to)346-354
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume436
Issue number2
DOIs
Publication statusPublished - Apr 15 2005

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Thylakoids
Acclimatization
Cyanobacteria
Shock
Hot Temperature
Lipids
Light
Synechocystis
Membranes
Photosynthesis
Liposomes
Anisotropy
Monolayers
Stabilization
Monitoring
Temperature
Experiments

Keywords

  • Heat stress lipid
  • Membrane reorganization
  • Stress protein
  • Thermal stability of membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

"Heat shock lipid" in cyanobacteria during heat/light-acclimation. / Balogi, Zsolt; Török, Z.; Balogh, G.; Jósvay, Katalin; Shigapova, Natalia; Vierling, Elizabeth; Vígh, L.; Horváth, I.

In: Archives of Biochemistry and Biophysics, Vol. 436, No. 2, 15.04.2005, p. 346-354.

Research output: Contribution to journalArticle

Balogi, Zsolt ; Török, Z. ; Balogh, G. ; Jósvay, Katalin ; Shigapova, Natalia ; Vierling, Elizabeth ; Vígh, L. ; Horváth, I. / "Heat shock lipid" in cyanobacteria during heat/light-acclimation. In: Archives of Biochemistry and Biophysics. 2005 ; Vol. 436, No. 2. pp. 346-354.
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