Heat induced susceptibility of barley lines with various types of resistance genes to powdery mildew

B. Barna, B. D. Harrach, O. Viczián, J. Fodor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Heat shock treatment of near isogenic barley lines induced susceptibility against powdery mildew (Blumeria graminis f. sp. hordei, Bgh). When barley lines were immersed into hot water (48-49 °C) for 20 seconds one day before inoculation with Bgh race A6, the heat treatment increased susceptibility in susceptible barley cv. Ingrid and in its near-isogenic barley lines carrying different effective resistance genes. Microscopic investigations indicated vigorous development of the pathogen not only on heat treated susceptible Ingrid and resistant Mla, but also on Mlg-resistant and even mlo-resistant lines. However, when longer heat stress was used, infection density increased gradually on the susceptible Ingrid leaves, and the 40-50 sec heat treatment induced the development of visible powdery mildew colonies even on mlo leaves. Heat stress significantly increased leakage of ions from leaf segments from all barley lines with or without specific resistance genes and caused a late decrease of SOD and a slight increase in CAT enzyme activities, which correlated with the slightly down-regulated levels of hydrogen peroxide in the heat treated barley leaves. Significant increase of RNase activities was found after heat stress, and there was a slight degradation of total DNA as a consequence of heat pretreatment in all barley lines.

Original languageEnglish
Pages (from-to)177-188
Number of pages12
JournalActa Phytopathologica et Entomologica Hungarica
Volume49
Issue number2
DOIs
Publication statusPublished - Dec 1 2014

Fingerprint

powdery mildew
barley
heat
heat stress
genes
leaves
heat treatment
Blumeria graminis f. sp. hordei
hydrogen peroxide
superoxide dismutase
pretreatment
enzyme activity
ions
degradation
pathogens
DNA
infection
water

Keywords

  • CAT and RNase enzymes
  • Heat stress
  • Ion leakage
  • Microscopy
  • Mla
  • Mlg and mlo genes
  • SOD

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

Heat induced susceptibility of barley lines with various types of resistance genes to powdery mildew. / Barna, B.; Harrach, B. D.; Viczián, O.; Fodor, J.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 49, No. 2, 01.12.2014, p. 177-188.

Research output: Contribution to journalArticle

@article{06570fca53174608a487fd03ef75fe06,
title = "Heat induced susceptibility of barley lines with various types of resistance genes to powdery mildew",
abstract = "Heat shock treatment of near isogenic barley lines induced susceptibility against powdery mildew (Blumeria graminis f. sp. hordei, Bgh). When barley lines were immersed into hot water (48-49 °C) for 20 seconds one day before inoculation with Bgh race A6, the heat treatment increased susceptibility in susceptible barley cv. Ingrid and in its near-isogenic barley lines carrying different effective resistance genes. Microscopic investigations indicated vigorous development of the pathogen not only on heat treated susceptible Ingrid and resistant Mla, but also on Mlg-resistant and even mlo-resistant lines. However, when longer heat stress was used, infection density increased gradually on the susceptible Ingrid leaves, and the 40-50 sec heat treatment induced the development of visible powdery mildew colonies even on mlo leaves. Heat stress significantly increased leakage of ions from leaf segments from all barley lines with or without specific resistance genes and caused a late decrease of SOD and a slight increase in CAT enzyme activities, which correlated with the slightly down-regulated levels of hydrogen peroxide in the heat treated barley leaves. Significant increase of RNase activities was found after heat stress, and there was a slight degradation of total DNA as a consequence of heat pretreatment in all barley lines.",
keywords = "CAT and RNase enzymes, Heat stress, Ion leakage, Microscopy, Mla, Mlg and mlo genes, SOD",
author = "B. Barna and Harrach, {B. D.} and O. Viczi{\'a}n and J. Fodor",
year = "2014",
month = "12",
day = "1",
doi = "10.1556/APhyt.49.2014.2.4",
language = "English",
volume = "49",
pages = "177--188",
journal = "Acta Phytopathologica et Entomologica Hungarica",
issn = "0238-1249",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Heat induced susceptibility of barley lines with various types of resistance genes to powdery mildew

AU - Barna, B.

AU - Harrach, B. D.

AU - Viczián, O.

AU - Fodor, J.

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Heat shock treatment of near isogenic barley lines induced susceptibility against powdery mildew (Blumeria graminis f. sp. hordei, Bgh). When barley lines were immersed into hot water (48-49 °C) for 20 seconds one day before inoculation with Bgh race A6, the heat treatment increased susceptibility in susceptible barley cv. Ingrid and in its near-isogenic barley lines carrying different effective resistance genes. Microscopic investigations indicated vigorous development of the pathogen not only on heat treated susceptible Ingrid and resistant Mla, but also on Mlg-resistant and even mlo-resistant lines. However, when longer heat stress was used, infection density increased gradually on the susceptible Ingrid leaves, and the 40-50 sec heat treatment induced the development of visible powdery mildew colonies even on mlo leaves. Heat stress significantly increased leakage of ions from leaf segments from all barley lines with or without specific resistance genes and caused a late decrease of SOD and a slight increase in CAT enzyme activities, which correlated with the slightly down-regulated levels of hydrogen peroxide in the heat treated barley leaves. Significant increase of RNase activities was found after heat stress, and there was a slight degradation of total DNA as a consequence of heat pretreatment in all barley lines.

AB - Heat shock treatment of near isogenic barley lines induced susceptibility against powdery mildew (Blumeria graminis f. sp. hordei, Bgh). When barley lines were immersed into hot water (48-49 °C) for 20 seconds one day before inoculation with Bgh race A6, the heat treatment increased susceptibility in susceptible barley cv. Ingrid and in its near-isogenic barley lines carrying different effective resistance genes. Microscopic investigations indicated vigorous development of the pathogen not only on heat treated susceptible Ingrid and resistant Mla, but also on Mlg-resistant and even mlo-resistant lines. However, when longer heat stress was used, infection density increased gradually on the susceptible Ingrid leaves, and the 40-50 sec heat treatment induced the development of visible powdery mildew colonies even on mlo leaves. Heat stress significantly increased leakage of ions from leaf segments from all barley lines with or without specific resistance genes and caused a late decrease of SOD and a slight increase in CAT enzyme activities, which correlated with the slightly down-regulated levels of hydrogen peroxide in the heat treated barley leaves. Significant increase of RNase activities was found after heat stress, and there was a slight degradation of total DNA as a consequence of heat pretreatment in all barley lines.

KW - CAT and RNase enzymes

KW - Heat stress

KW - Ion leakage

KW - Microscopy

KW - Mla

KW - Mlg and mlo genes

KW - SOD

UR - http://www.scopus.com/inward/record.url?scp=84922571840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922571840&partnerID=8YFLogxK

U2 - 10.1556/APhyt.49.2014.2.4

DO - 10.1556/APhyt.49.2014.2.4

M3 - Article

VL - 49

SP - 177

EP - 188

JO - Acta Phytopathologica et Entomologica Hungarica

JF - Acta Phytopathologica et Entomologica Hungarica

SN - 0238-1249

IS - 2

ER -