Hatching out goldfish from common carp eggs

Interspecific androgenesis between two cyprinid species

Miklós Bercsényi, István Magyary, B. Urbányi, László Orbán, László Horváth

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

We have successfully performed interspecific androgenesis between two cyprinid species. Gamma-ray irradiated eggs of common carp were fertilized with fresh and cryopreserved sperm of three different goldfish varieties and the haploid embryos were then heat-shocked to restore diploidy and to produce viable offspring. Androgenic diploid goldfish progenies from over a dozen different experiments were screened for four phenotypic markers several times. Color and other phenotypic markers characteristic of goldfish were found exclusively among androgenetic goldfish progenies; no markers originating from common carp were detected in over 1503 individuals investigated visually. RAPD assay was used to compare the parents and the offspring at the genomic level. The RAPD pattern of the androgenetic goldfish contained exclusively paternal bands, thereby confirming the results of the phenotypic analysis. According to our knowledge, this is the first successful interspecific androgenesis performed with two different species resulting in viable offspring.

Original languageEnglish
Pages (from-to)573-579
Number of pages7
JournalGenome
Volume41
Issue number4
Publication statusPublished - 1998

Fingerprint

androgenesis
Goldfish
Carps
goldfish
Cyprinus carpio
Eggs
hatching
Diploidy
diploidy
Haploidy
Gamma Rays
haploidy
gamma radiation
Spermatozoa
embryo (animal)
Embryonic Structures
Color
Hot Temperature
spermatozoa
genomics

Keywords

  • Nuclear-mitochondrial incompatibility
  • RAPD
  • Whole genome manipulation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Bercsényi, M., Magyary, I., Urbányi, B., Orbán, L., & Horváth, L. (1998). Hatching out goldfish from common carp eggs: Interspecific androgenesis between two cyprinid species. Genome, 41(4), 573-579.

Hatching out goldfish from common carp eggs : Interspecific androgenesis between two cyprinid species. / Bercsényi, Miklós; Magyary, István; Urbányi, B.; Orbán, László; Horváth, László.

In: Genome, Vol. 41, No. 4, 1998, p. 573-579.

Research output: Contribution to journalArticle

Bercsényi, M, Magyary, I, Urbányi, B, Orbán, L & Horváth, L 1998, 'Hatching out goldfish from common carp eggs: Interspecific androgenesis between two cyprinid species', Genome, vol. 41, no. 4, pp. 573-579.
Bercsényi, Miklós ; Magyary, István ; Urbányi, B. ; Orbán, László ; Horváth, László. / Hatching out goldfish from common carp eggs : Interspecific androgenesis between two cyprinid species. In: Genome. 1998 ; Vol. 41, No. 4. pp. 573-579.
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