Haploid regeneration aptitude of maize (Zea mays L.) lines of various origin and of their hybrids

T. Spitkó, L. Sági, J. Pintér, L. C. Marton, B. Barnabás

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

One maize line with good haploid regeneration aptitude and one recalcitrant in this respect, together with various hybrid combinations of these, were used to develop new doubled haploid (DH) lines. A line originating from an exotic Chinese stock was crossed with a recalcitrant Martonvásár line, used as a parental component in several commercial hybrids, to determine the anther response, callus induction and plant regeneration of the reciprocal hybrids and the fertility of the adult plants. The same tests were also made on previously developed DH lines of these reciprocal hybrids and on DH lines obtained after a single backcross with the recalcitrant parent. The results indicated that haploid regeneration aptitude was primarily an inherited trait in the experimental material, with a heterosis effect in the F 1 progeny. The hybrids exhibited no statistically significant reciprocal effect. The anther response, callus induction and plant regeneration of the DH lines developed from the hybrids and the fertility of regenerated plants were comparable to the initial combinations even when the DH line originated from a hybrid developed by backcrossing with the recalcitrant line. Data are also reported on the anther response and haploid regeneration frequency of genotypes known to have excellent tissue culturing aptitude from immature zygotic embryos. The present results may contribute to the development of an optimum gene ratio where, in addition to maintaining haploid regeneration aptitude, the DH lines also possess important agronomic traits, which are then reliably inherited.

Original languageEnglish
Pages (from-to)537-542
Number of pages6
JournalMaydica
Volume51
Issue number3-4
Publication statusPublished - 2006

Fingerprint

Haploidy
haploidy
doubled haploids
Zea mays
Regeneration
corn
anthers
callus
Bony Callus
plant fertility
Fertility
backcrossing
mature plants
heterosis
agronomic traits
embryo (plant)
Hybrid Vigor
tissue culture
Inbreeding
immatures

Keywords

  • Anther culture
  • Doubled haploid
  • Line development
  • Maize
  • Plant regeneration

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Haploid regeneration aptitude of maize (Zea mays L.) lines of various origin and of their hybrids. / Spitkó, T.; Sági, L.; Pintér, J.; Marton, L. C.; Barnabás, B.

In: Maydica, Vol. 51, No. 3-4, 2006, p. 537-542.

Research output: Contribution to journalArticle

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AU - Sági, L.

AU - Pintér, J.

AU - Marton, L. C.

AU - Barnabás, B.

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