Handheld nonlinear microscope system comprising a 2 MHz repetition rate, modelocked YB-fiber laser for in vivo biomedical imaging

Krolopp, Attila Csákányi, Dóra Haluszka, Dániel Csáti, Lajos Vass, Attila Kolonics, N. Wikonkál, R. Szipócs

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A novel, Yb-fiber laser based, handheld 2PEF/SHG microscope imaging system is introduced. It is suitable for in vivo imaging of murine skin at an average power level as low as 5 mW at 200 kHz sampling rate. Amplified and compressed laser pulses having a spectral bandwidth of 8 to 12 nm at around 1030 nm excite the biological samples at a ~1.89 MHz repetition rate, which explains how the high quality two-photon excitation fluorescence (2PEF) and second harmonic generation (SHG) images are obtained at the average power level of a laser pointer. The scanning, imaging and detection head, which comprises a conventional microscope objective for beam focusing, has a physical length of ~180 mm owing to the custom designed imaging telescope system between the laser scanner mirrors and the entrance aperture of the microscope objective. Operation of the all-fiber, all-normal dispersion Yb-fiber ring laser oscillator is electronically controlled by a two-channel polarization controller for Q-switching free mode-locked operation. The whole nonlinear microscope imaging system has the main advantages of the low price of the fs laser applied, fiber optics flexibility, a relatively small, light-weight scanning and detection head, and a very low risk of thermal or photochemical damage of the skin samples.

Original languageEnglish
Article number#269667
Pages (from-to)3531-3542
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 1 2016

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fiber lasers
repetition
Lasers
microscopes
lasers
harmonic generations
dials
scanning
ring lasers
Head
entrances
scanners
Telescopes
fiber optics
controllers
Skin
flexibility
apertures
sampling
oscillators

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Handheld nonlinear microscope system comprising a 2 MHz repetition rate, modelocked YB-fiber laser for in vivo biomedical imaging. / Krolopp; Csákányi, Attila; Haluszka, Dóra; Csáti, Dániel; Vass, Lajos; Kolonics, Attila; Wikonkál, N.; Szipócs, R.

In: Biomedical Optics Express, Vol. 7, No. 9, #269667, 01.09.2016, p. 3531-3542.

Research output: Contribution to journalArticle

Krolopp ; Csákányi, Attila ; Haluszka, Dóra ; Csáti, Dániel ; Vass, Lajos ; Kolonics, Attila ; Wikonkál, N. ; Szipócs, R. / Handheld nonlinear microscope system comprising a 2 MHz repetition rate, modelocked YB-fiber laser for in vivo biomedical imaging. In: Biomedical Optics Express. 2016 ; Vol. 7, No. 9. pp. 3531-3542.
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